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dc.contributor.authorBonelo, A
dc.contributor.authorValmori, D
dc.contributor.authorTriponez, F
dc.contributor.authorTiercy, JM
dc.contributor.authorMentha, G
dc.contributor.authorOberholzer, J
dc.contributor.authorChampagne, P
dc.contributor.authorRomero, JF
dc.contributor.authorEsposito, F
dc.contributor.authorNebie, I
dc.contributor.authorBarbey, C
dc.contributor.authorRomero, P
dc.contributor.authorHerrera, S
dc.contributor.authorCorradin, G
dc.contributor.authorLopez, JA
dc.date.accessioned2011-05-10
dc.date.accessioned2013-12-19T22:58:00Z
dc.date.accessioned2017-03-02T00:42:19Z
dc.date.available2017-03-02T00:42:19Z
dc.date.issued2000
dc.date.modified2013-12-19T22:58:00Z
dc.identifier.issn0014-2980
dc.identifier.doi10.1002/1521-4141(200011)30:11<3079::AID-IMMU3079>3.0.CO;2-7
dc.identifier.urihttp://hdl.handle.net/10072/55245
dc.description.abstractCD8+ cytolytic T lymphocytes (CTL) play a fundamental role in the clearance of malaria parasites from the liver in mouse models. In humans, however, only low levels of parasite-specific CD8+ T lymphocytes have been observed in individuals living in endemic areas. In the present study, we identified high levels of circulating CD8+ T lymphocytes specific for a previously described HLA-A2-restricted CTL epitope of the circumsporozoite (CS) protein of Plasmodium falciparum in an adult living in Burkina Faso, as evidenced by IFN- + ELISPOT assay and MHC-tetramer technology. After cloning by limiting dilution culture, T cell recognition of natural CS variants of P. falciparum was studied. The results demonstrate that naturally occurring variations drastically affect residues critical for T cell recognition as only two out of nine sequences analyzed were efficiently recognized by the CTL clones. These clones were also used to analyze T cell recognition of the endogenously presented cognate antigen. We observed efficient antigen recognition of both HLA-A*0201-transfected murine antigen presenting cells and liver cells from HLA-A*0201/Kb-transgenic mice upon infection with recombinant vaccinia virus encoding the CS protein (WR-CS). More importantly, we demonstrate for the first time efficient recognition of WR-CS-infected human liver cells.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherWiley-VCH
dc.publisher.placeWeihheim, Germany
dc.relation.ispartofpagefrom3079
dc.relation.ispartofpageto3088
dc.relation.ispartofissue11
dc.relation.ispartofjournalEuropean Journal of Immunology
dc.relation.ispartofvolume30
dc.subject.fieldofresearchCellular Immunology
dc.subject.fieldofresearchImmunology
dc.subject.fieldofresearchcode110704
dc.subject.fieldofresearchcode1107
dc.titleGeneration and characterization of malaria specific human CD8+ lymphocyte clones: Effect of natural polymorphism on T cell recognition and on the recognition of cognate antigen endogenously presented by liver cells
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codec1x
gro.facultyFaculty of Science, Environment, Engineering and Technology
gro.date.issued2000
gro.hasfulltextNo Full Text
gro.griffith.authorLopez Ramirez, Alejandro


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