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dc.contributor.authorArora, Devinder S
dc.contributor.authorNimitvilai, Sudarat
dc.contributor.authorTeppen, Tara L
dc.contributor.authorMcElvain, Maureen A
dc.contributor.authorSakharkar, Amul J
dc.contributor.authorYou, Chang
dc.contributor.authorPandey, Subhash C
dc.contributor.authorBrodie, Mark S
dc.date.accessioned2017-05-03T16:12:39Z
dc.date.available2017-05-03T16:12:39Z
dc.date.issued2013
dc.date.modified2014-01-08T05:15:55Z
dc.identifier.issn0893-133X
dc.identifier.doi10.1038/npp.2013.65
dc.identifier.urihttp://hdl.handle.net/10072/55370
dc.description.abstractPutative dopaminergic (pDAergic) ventral tegmental area (VTA) neurons have an important role in alcohol addiction. Acute ethanol increases the activity of pDAergic neurons, and withdrawal from repeated ethanol administration produces a decreased sensitivity of pDAergic VTA neurons to GABA. Recent studies show that behavioral changes induced by chronic alcohol are reversed by inhibitors of histone deacetylases (HDACs). Whether HDAC-induced histone modifications regulate changes in GABA sensitivity of VTA pDAergic neurons during withdrawal is unknown. Here, we investigated modulation of withdrawal-induced changes in GABA sensitivity of pDAergic VTA neurons by HDAC inhibitors (HDACi), and also measured the levels of HDAC2, histone (H3-K9) acetylation, and GABA-Aa1 receptor (GABA (A-a1) R) subunit in VTA during ethanol withdrawal. Mice were injected intraperitoneally (ip) with either ethanol (3.5 g/kg) or saline twice daily for 3 weeks. In recordings from pDAergic VTA neurons in brain slices from ethanol-withdrawn mice, sensitivity to GABA (50-500 卩 was reduced. In brain slices from ethanol-withdrawn mice incubated with the HDACi SAHA (vorinostat) or trichostatin A (TSA) for 2 h, the hyposensitivity of pDAergic VTA neurons to GABA was significantly attenuated. There was no effect of TSA or SAHA on GABA sensitivity of pDAergic VTA neurons from saline-treated mice. In addition, ethanol withdrawal was associated with an increase in levels of HDAC2 and a decrease in histone (H3-K9) acetylation and levels of GABA (A-a1) R subunits in the VTA. Therefore, blockade of upregulation of HDAC2 by HDACi normalizes GABA hyposensitivity of pDAergic neurons developed during withdrawal after chronic ethanol treatment, which suggests the possibility that inhibition of HDACs can reverse ethanol-induced neuroadaptational changes in reward circuitry.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherNature Publishing Group
dc.publisher.placeUnited Kingdom
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom1674
dc.relation.ispartofpageto1684
dc.relation.ispartofissue9
dc.relation.ispartofjournalNeuropsychopharmacology
dc.relation.ispartofvolume38
dc.rights.retentionY
dc.subject.fieldofresearchCentral Nervous System
dc.subject.fieldofresearchMedical and Health Sciences
dc.subject.fieldofresearchPsychology and Cognitive Sciences
dc.subject.fieldofresearchcode110903
dc.subject.fieldofresearchcode11
dc.subject.fieldofresearchcode17
dc.titleHyposensitivity to Gamma-Aminobutyric Acid in the Ventral Tegmental Area During Alcohol Withdrawal: Reversal by Histone Deacetylase Inhibitors
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2013
gro.hasfulltextNo Full Text
gro.griffith.authorArora, Devinder S.


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