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dc.contributor.authorOwen, Suzzanne J
dc.contributor.authorMassa, Helen M
dc.contributor.authorRose'Meyer, Roselyn B
dc.date.accessioned2017-05-03T12:55:09Z
dc.date.available2017-05-03T12:55:09Z
dc.date.issued2013
dc.date.modified2014-01-13T00:48:19Z
dc.identifier.issn0028-1298
dc.identifier.doi10.1007/s00210-013-0892-x
dc.identifier.urihttp://hdl.handle.net/10072/55583
dc.description.abstractThis study examined the effect of age, ovariectomy and dietary phytoestrogen ingestion on 17-߭estradiol-mediated relaxant responses and messenger RNA (mRNA) and protein expression of oestrogen receptor subtypes in the rat isolated bladder. Female Wistar rats (8 weeks) were anaesthetised, and the ovaries were removed (ovx) or left intact (sham). Rats were fed either normal rat chow (soy, phytoestrogens) or a non-soy (phytoestrogen free) diet. Isolated bladder from rats aged 12, 24 or 52 weeks were pre-contracted with 3 占carbachol prior to obtaining a concentration response curve to 17-߭estradiol. Protein and mRNA expression of the oestrogen receptor subtypes was completed using immunohistochemistry and real-time PCR, respectively. Relatively moderate relaxant responses to 17-߭estradiol were observed in bladders from all age and treatment groups. However, in soy-fed sham 52-week-old rats, the bladder exhibited enhanced relaxant responses to 17-߭estradiol when compared to tissues from other age-matched rat treatment groups (P?<?0.05). In bladders from female rats, the mRNA and protein expression of oestrogen receptors ߠwas significantly greater than the expression of the oestrogen receptor a. Oestrogen receptor a mRNA expression declined with age (P?<?0.05), whereas oestrogen receptor ߠexpression did not change in any of the treatment groups (P?>?0.05). Diet, overiectomy or age did not alter the protein expression of either oestrogen receptor subtype in the bladder (P?>?0.05). While a soy diet improved relaxant effects to the 17-߭estradiol with age, it did not alter relaxant responses in bladders from ovariectomised rats.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSpringer Verlag
dc.publisher.placeGermany
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom917
dc.relation.ispartofpageto928
dc.relation.ispartofissue10
dc.relation.ispartofjournalNaunyn-Schmiedeberg Archives of Pharmacology
dc.relation.ispartofvolume386
dc.rights.retentionY
dc.subject.fieldofresearchPharmacology and pharmaceutical sciences
dc.subject.fieldofresearchBasic pharmacology
dc.subject.fieldofresearchMedical physiology
dc.subject.fieldofresearchcode3214
dc.subject.fieldofresearchcode321401
dc.subject.fieldofresearchcode3208
dc.titleDietary Phytoestrogen improves relaxant responses to 17 –β-estradiol in aged but not ovariectomised rat bladders
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Health, School of Nursing and Midwifery
gro.date.issued2013
gro.hasfulltextNo Full Text
gro.griffith.authorMassa, Helen M.
gro.griffith.authorOwen, Suzzanne J.
gro.griffith.authorRose'Meyer, Roselyn B.


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