Show simple item record

dc.contributor.authorJing, Jing
dc.contributor.authorRen, Wei C
dc.contributor.authorLi, Chun
dc.contributor.authorBose, Utpal
dc.contributor.authorParekh, Harendra S
dc.contributor.authorWei, Ming Q
dc.date.accessioned2017-05-03T15:30:10Z
dc.date.available2017-05-03T15:30:10Z
dc.date.issued2013
dc.date.modified2014-01-15T21:48:23Z
dc.identifier.issn0269-3879
dc.identifier.doi10.1002/bmc.2858
dc.identifier.urihttp://hdl.handle.net/10072/55629
dc.description.abstractoad parotoid gland secretion or toad venom has in recent years been increasingly shown to possess potentially beneficial pharmacological effects; this speculation has drawn much interest centred on elucidating the chemical basis of its multimodal effects. For this purpose, we explored the use of a rapid and accurate analysis method for systemic investigation of the parotoid gland chemistry, when extracted from Australian cane toads. Full-scan data of cane toad venom extract was acquired using high-performance liquid chromatography coupled with a hybrid quadrupole-time of flight mass spectrometry system (HPLC/MS-Q-TOF), with multiple ionization sources (ESI and APCI) in positive and negative mixed modes. By measuring the exact mass differences between the theoretical and measured mass of each assumed compound, we confirmed the presence of 12 key constituents. The present results demonstrate that the use of HPLC/MS-Q-TOF with multiple ionization sources delivers exemplary selectivity and sensitivity, allowing for the rapid and accurate identification of constituents within cane toad venom. This paves the way for this technique to be used in future routine screening of components within the genus Bufo and for key analytes too, then reliably assessed for any purported beneficial (clinic) properties.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherJohn Wiley & Sons
dc.publisher.placeUnited Kingdom
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom685
dc.relation.ispartofpageto687
dc.relation.ispartofissue6
dc.relation.ispartofjournalBiomedical Chromatography
dc.relation.ispartofvolume27
dc.rights.retentionY
dc.subject.fieldofresearchAnalytical chemistry
dc.subject.fieldofresearchPharmacology and pharmaceutical sciences
dc.subject.fieldofresearchcode3401
dc.subject.fieldofresearchcode3214
dc.titleRapid identification of primary constituents in parotoid gland secretions of the Australian cane toad using HPLC/MS-Q-TOF
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Health, School of Medical Science
gro.date.issued2013
gro.hasfulltextNo Full Text
gro.griffith.authorWei, Ming Q.


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record