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dc.contributor.authorHummitzsch, Katja
dc.contributor.authorIrving-Rodgers, Helen F
dc.contributor.authorHatzirodos, Nicholas
dc.contributor.authorBonner, Wendy
dc.contributor.authorSabatier, Laetitia
dc.contributor.authorReinhardt, Dieter P
dc.contributor.authorSado, Yoshikazu
dc.contributor.authorNinomiya, Yoshifumi
dc.contributor.authorWilhelm, Dagmar
dc.contributor.authorRodgers, Raymond J
dc.date.accessioned2017-05-03T16:13:09Z
dc.date.available2017-05-03T16:13:09Z
dc.date.issued2013
dc.date.modified2014-01-17T04:18:22Z
dc.identifier.issn1932-6203
dc.identifier.doi10.1371/journal.pone.0055578
dc.identifier.urihttp://hdl.handle.net/10072/55754
dc.description.abstractOvarian follicular granulosa cells surround and nurture oocytes, and produce sex steroid hormones. It is believed that during development the ovarian surface epithelial cells penetrate into the ovary and develop into granulosa cells when associating with oogonia to form follicles. Using bovine fetal ovaries (n = 80) we identified a novel cell type, termed GREL for Gonadal Ridge Epithelial-Like. Using 26 markers for GREL and other cells and extracellular matrix we conducted immunohistochemistry and electron microscopy and chronologically tracked all somatic cell types during development. Before 70 days of gestation the gonadal ridge/ovarian primordium is formed by proliferation of GREL cells at the surface epithelium of the mesonephros. Primordial germ cells (PGCs) migrate into the ovarian primordium. After 70 days, stroma from the underlying mesonephros begins to penetrate the primordium, partitioning the developing ovary into irregularly-shaped ovigerous cords composed of GREL cells and PGCs/oogonia. Importantly we identified that the cords are always separated from the stroma by a basal lamina. Around 130 days of gestation the stroma expands laterally below the outermost layers of GREL cells forming a sub-epithelial basal lamina and establishing an epithelial-stromal interface. It is at this stage that a mature surface epithelium develops from the GREL cells on the surface of the ovary primordium. Expansion of the stroma continues to partition the ovigerous cords into smaller groups of cells eventually forming follicles containing an oogonium/oocyte surrounded by GREL cells, which become granulosa cells, all enclosed by a basal lamina. Thus in contrast to the prevailing theory, the ovarian surface epithelial cells do not penetrate into the ovary to form the granulosa cells of follicles, instead ovarian surface epithelial cells and granulosa cells have a common precursor, the GREL cell.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent8006948 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherPublic Library of Science
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrome55578-1
dc.relation.ispartofpagetoe55578-16
dc.relation.ispartofissue2
dc.relation.ispartofjournalPloS One
dc.relation.ispartofvolume8
dc.rights.retentionY
dc.subject.fieldofresearchAnimal physiology - cell
dc.subject.fieldofresearchZoology not elsewhere classified
dc.subject.fieldofresearchcode310909
dc.subject.fieldofresearchcode310999
dc.titleA New Model of Development of the Mammalian Ovary and Follicles
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://www.plos.org/journals/license.html
gro.rights.copyright© 2013 Hummitzsch et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License CCAL. (http://www.plos.org/journals/license.html) Need to email author
gro.date.issued2013
gro.hasfulltextFull Text
gro.griffith.authorIrving-Rodgers, Helen F.


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