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dc.contributor.authorKaarbo, Mari
dc.contributor.authorCrane, Denis I
dc.contributor.authorMurrell, Wayne G
dc.date.accessioned2017-05-03T11:02:04Z
dc.date.available2017-05-03T11:02:04Z
dc.date.issued2013
dc.date.modified2014-01-23T22:38:45Z
dc.identifier.issn1537-744X
dc.identifier.doi10.1155/2013/491546
dc.identifier.urihttp://hdl.handle.net/10072/56047
dc.description.abstractEarlier findings from our laboratory implicated RhoA in heart developmental processes. To investigate factors that potentially regulate RhoA expression, RhoA gene organisation and promoter activity were analysed. Comparative analysis indicated strict conservation of both gene organisation and coding sequence of the chick, mouse, and human RhoA genes. Bioinformatics analysis of the derived promoter region of mouse RhoA identified putative consensus sequence binding sites for several transcription factors involved in heart formation and organogenesis generally. Using luciferase reporter assays, RhoA promoter activity was shown to increase in mouse-derived P19CL6 cells that were induced to differentiate into cardiomyocytes. Overexpression of a dominant negative mutant of mouse RhoA (mRhoAN19) blocked this cardiomyocyte differentiation of P19CL6 cells and led to the accumulation of the cardiac transcription factors SRF and GATA4 and the early cardiac marker cardiac a-actin. Taken together, these findings indicate a fundamental role for RhoA in the differentiation of cardiomyocytes.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent4019513 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.publisherHindawi Publishing
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom491546-1
dc.relation.ispartofpageto491546-12
dc.relation.ispartofjournalThe Scientific World Journal
dc.relation.ispartofvolume2013
dc.rights.retentionY
dc.subject.fieldofresearchCell Development, Proliferation and Death
dc.subject.fieldofresearchcode060103
dc.titleRhoA Regulation of Cardiomyocyte Differentiation
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by-nc-sa/2.1/au/
gro.rights.copyright© The Author(s) 2013. The attached file is posted here with permission of the copyright owners for your personal use only. No further distribution permitted.For information about this journal please refer to the journal’s website. The online version of this work is licensed under a Creative Commons License, available at http://creativecommons.org/licenses/by-nc-sa/2.1/au/
gro.date.issued2013
gro.hasfulltextFull Text
gro.griffith.authorCrane, Denis I.


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