Show simple item record

dc.contributor.authorRuhayel, Rasha A
dc.contributor.authorBerners-Price, Susan J
dc.contributor.authorFarrell, Nicholas P
dc.date.accessioned2018-03-20T12:30:26Z
dc.date.available2018-03-20T12:30:26Z
dc.date.issued2013
dc.date.modified2014-01-29T23:05:00Z
dc.identifier.issn1477-9226
dc.identifier.doi10.1039/C2DT32079G
dc.identifier.urihttp://hdl.handle.net/10072/56145
dc.description.abstract2D [1H, 15N] HSQC NMR spectroscopy has been used to monitor the reaction of fully 15N-labelled [{trans-PtCl(NH3)2}2(μ-trans-Pt(NH3)2{NH2(CH2)6NH2}2)]4+ (Triplatin, BBR3464 or 1,0,1/t,t,t (15N-1)) with the self-complementary 10-mer DNA duplex 5′-{d(ACGTATACGT)2} (duplex I) at pH 5.4 and 298 K. Initial electrostatic interactions were observed in the minor groove of the duplex, followed directly by aquation to form the monoaqua monochloro species. There was evidence for two discrete monofunctional adducts, through covalent binding at the guanine N7 sites, and one had distinctly different 1H/15N chemical shifts to those observed previously in analogous reactions. Bifunctional adduct formation followed by binding at a second guanine N7 site with evidence for both the 3′–3′ 1,2-GG and 5′–5′ 1,6-GG interstrand cross-links in a ratio of 2 : 1. The results show that cross-link preference is kinetically controlled and will depend critically on the reaction conditions, explaining why in a previous reaction of 1 with duplex I the major adduct isolated by HPLC had two simultaneous 3′–3′ 1,2-interstrand cross-links.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherRSC Publishing
dc.publisher.placeUnited Kingdom
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom3181
dc.relation.ispartofpageto3187
dc.relation.ispartofissue9
dc.relation.ispartofjournalDalton Transactions
dc.relation.ispartofvolume42
dc.relation.urihttp://purl.org/au-research/grants/ARC/DP1095383
dc.relation.grantIDDP1095383
dc.relation.fundersARC
dc.rights.retentionY
dc.subject.fieldofresearchBioinorganic Chemistry
dc.subject.fieldofresearchInorganic Chemistry
dc.subject.fieldofresearchTheoretical and Computational Chemistry
dc.subject.fieldofresearchOther Chemical Sciences
dc.subject.fieldofresearchcode030201
dc.subject.fieldofresearchcode0302
dc.subject.fieldofresearchcode0307
dc.subject.fieldofresearchcode0399
dc.titleCompetitive formation of both long-range 5'-5' and short-range antiparallel 3'- 3' DNA interstrand cross-links by a trinuclear platinum complex on binding to a 10-mer duplex
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dc.description.versionAccepted Manuscript (AM)
gro.rights.copyright© 2013 Royal Society of Chemistry. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal website for access to the definitive, published version.
gro.date.issued2013
gro.hasfulltextFull Text
gro.griffith.authorBerners-Price, Sue J.
gro.griffith.authorRuhayel, Rasha
gro.griffith.authorFarrell, Nicholas


Files in this item

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record