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dc.contributor.authorGraham, Clara M
dc.contributor.authorNarayan, Edward J
dc.contributor.authorMcCallum, Hamish
dc.contributor.authorHero, Jean-Marc
dc.date.accessioned2017-05-03T15:44:20Z
dc.date.available2017-05-03T15:44:20Z
dc.date.issued2013
dc.date.modified2014-01-29T23:23:24Z
dc.identifier.issn0016-6480
dc.identifier.doi10.1016/j.ygcen.2013.05.012
dc.identifier.urihttp://hdl.handle.net/10072/56166
dc.description.abstractThis study used non-invasive endocrinology to examine baseline corticosterone at different altitudes in a free-living Australian amphibian: the Great Barred Frog (Mixophyes fasciolatus). An enzyme immunoassay (EIA) was performed on urine samples and validated biologically using an adrenocorticotropic hormone (ACTH) challenge. Frogs were injected with ACTH on day 0 and recaptures occurred 1-10 days post injection. Urine samples and body condition measurements were collected from lowland (60 m) and highland (660 m and 790 m) sub-populations of M. fasciolatus in South East Queensland (SEQ), close to their post-breeding period during autumn 2011. We simultaneously sampled these sub-populations for Batrachochytrium dendrobatidis (Bd), a pathogenic fungus responsible for mass mortalities of amphibians worldwide. The ACTH challenge successfully validated the urinary corticosterone EIA in M. fasciolatus, with a peak urinary corticosterone response to ACTH injection on day 2 and a return to baseline levels by day 6. Polymerase chain reaction (PCR) analysis of 50 individuals returned only 1 positive result for Bd. Simple linear regression showed a strong positive relationship between baseline urinary corticosterone concentrations and altitude and no relationship with body condition. We hypothesize that higher baseline corticosterone concentrations within highland sub-populations of male M. fasciolatus could be associated with increased environmental challenge at high altitudes and geographic range limits. Whether this pattern is an indication of chronic stress in highland populations or life-time fitness and survival, warrants future investigation.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent608667 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationY
dc.relation.ispartofpagefrom24
dc.relation.ispartofpageto30
dc.relation.ispartofjournalGeneral and Comparative Endocrinology
dc.relation.ispartofvolume191
dc.rights.retentionY
dc.subject.fieldofresearchEcological Physiology
dc.subject.fieldofresearchEcological Impacts of Climate Change
dc.subject.fieldofresearchEcosystem Function
dc.subject.fieldofresearchPhysiology
dc.subject.fieldofresearchZoology
dc.subject.fieldofresearchVeterinary Sciences
dc.subject.fieldofresearchcode060203
dc.subject.fieldofresearchcode050101
dc.subject.fieldofresearchcode050102
dc.subject.fieldofresearchcode0606
dc.subject.fieldofresearchcode0608
dc.subject.fieldofresearchcode0707
dc.titleNon-invasive monitoring of glucocorticoid physiology within highland and lowland populations of native Australian Great Barred Frog (Mixophyes fasciolatus)
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Sciences, Griffith School of Environment
gro.rights.copyright© 2013 Elsevier. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
gro.date.issued2013
gro.hasfulltextFull Text
gro.griffith.authorHero, Jean-Marc
gro.griffith.authorGraham, Clara
gro.griffith.authorMcCallum, Hamish
gro.griffith.authorNarayan, Edward J.


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