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  • Isolation and characterization of 21 polymorphic microsatellite loci in the iconic Australian lungfish, Neoceratodus forsteri, using the Ion Torrent next-generation sequencing platform

    Author(s)
    Huey, Joel A
    Real, Kathryn M
    Mather, Peter B
    Chand, Vincent
    Roberts, David T
    Espinoza, Thomas
    McDougall, Andrew
    Kind, Peter K
    Brooks, Steven
    Hughes, Jane M
    Griffith University Author(s)
    Hughes, Jane M.
    Huey, Joel A.
    Real, Kathryn M.
    Roberts, David T.
    Year published
    2013
    Metadata
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    Abstract
    We isolated and characterized 21 microsatellite loci in the vulnerable and iconic Australian lungfish, Neoceratodus forsteri. Loci were screened across eight individuals from the Burnett River and 40 individuals from the Pine River. Genetic diversity was low with between one and six alleles per locus within populations and a maximum expected heterozygosity of 0.774. These loci will now be available to assess effective population sizes and genetic structure in N. forsteri across its natural range in South East Queensland, Australia.We isolated and characterized 21 microsatellite loci in the vulnerable and iconic Australian lungfish, Neoceratodus forsteri. Loci were screened across eight individuals from the Burnett River and 40 individuals from the Pine River. Genetic diversity was low with between one and six alleles per locus within populations and a maximum expected heterozygosity of 0.774. These loci will now be available to assess effective population sizes and genetic structure in N. forsteri across its natural range in South East Queensland, Australia.
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    Journal Title
    Conservation Genetics Resources
    Volume
    5
    DOI
    https://doi.org/10.1007/s12686-013-9894-8
    Subject
    Freshwater Ecology
    Evolutionary Biology
    Genetics
    Fisheries Sciences
    Publication URI
    http://hdl.handle.net/10072/56209
    Collection
    • Journal articles

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