Isolation and characterization of 21 polymorphic microsatellite loci in the iconic Australian lungfish, Neoceratodus forsteri, using the Ion Torrent next-generation sequencing platform
Author(s)
Huey, Joel A
Real, Kathryn M
Mather, Peter B
Chand, Vincent
Roberts, David T
Espinoza, Thomas
McDougall, Andrew
Kind, Peter K
Brooks, Steven
Hughes, Jane M
Griffith University Author(s)
Year published
2013
Metadata
Show full item recordAbstract
We isolated and characterized 21 microsatellite loci in the vulnerable and iconic Australian lungfish, Neoceratodus forsteri. Loci were screened across eight individuals from the Burnett River and 40 individuals from the Pine River. Genetic diversity was low with between one and six alleles per locus within populations and a maximum expected heterozygosity of 0.774. These loci will now be available to assess effective population sizes and genetic structure in N. forsteri across its natural range in South East Queensland, Australia.We isolated and characterized 21 microsatellite loci in the vulnerable and iconic Australian lungfish, Neoceratodus forsteri. Loci were screened across eight individuals from the Burnett River and 40 individuals from the Pine River. Genetic diversity was low with between one and six alleles per locus within populations and a maximum expected heterozygosity of 0.774. These loci will now be available to assess effective population sizes and genetic structure in N. forsteri across its natural range in South East Queensland, Australia.
View less >
View less >
Journal Title
Conservation Genetics Resources
Volume
5
Subject
Freshwater ecology
Evolutionary biology
Genetics
Fisheries sciences