dc.contributor.author | Good, Michael F | |
dc.contributor.author | Reiman, Jennifer M | |
dc.contributor.author | Rodriguez, I Bibiana | |
dc.contributor.author | Ito, Koichi | |
dc.contributor.author | Yanow, Stephanie K | |
dc.contributor.author | El-Deeb, Ibrahim M | |
dc.contributor.author | Batzloff, Michael R | |
dc.contributor.author | Stanisic, Danielle I | |
dc.contributor.author | Engwerda, Christian | |
dc.contributor.author | Spithill, Terry | |
dc.contributor.author | Hoffman, Stephen L | |
dc.contributor.author | Lee, Moses | |
dc.contributor.author | McPhun, Virginia | |
dc.date.accessioned | 2017-05-03T12:53:56Z | |
dc.date.available | 2017-05-03T12:53:56Z | |
dc.date.issued | 2013 | |
dc.date.modified | 2014-01-30T22:37:17Z | |
dc.identifier.issn | 0021-9738 | |
dc.identifier.doi | 10.1172/JCI66634 | |
dc.identifier.uri | http://hdl.handle.net/10072/56258 | |
dc.description.abstract | Vaccine development for the blood stages of malaria has focused on the induction of antibodies to parasite surface antigens, most of which are highly polymorphic. An alternate strategy has evolved from observations that low-density infections can induce antibody-independent immunity to different strains. To test this strategy, we treated parasitized red blood cells from the rodent parasite Plasmodium chabaudi with seco-cyclopropyl pyrrolo indole analogs. These drugs irreversibly alkylate parasite DNA, blocking their ability to replicate. After administration in mice, DNA from the vaccine could be detected in the blood for over 110 days and a single vaccination induced profound immunity to different malaria parasite species. Immunity was mediated by CD4+ T cells and was dependent on the red blood cell membrane remaining intact. The human parasite, Plasmodium falciparum, could also be attenuated by treatment with seco-cyclopropyl pyrrolo indole analogs. These data demonstrate that vaccination with chemically attenuated parasites induces protective immunity and provide a compelling rationale for testing a blood-stage parasite-based vaccine targeting human Plasmodium species. | |
dc.description.peerreviewed | Yes | |
dc.description.publicationstatus | Yes | |
dc.format.extent | 1840388 bytes | |
dc.format.mimetype | application/pdf | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | American Society for Clinical Investigation | |
dc.publisher.place | United States | |
dc.relation.ispartofstudentpublication | N | |
dc.relation.ispartofpagefrom | 3353 | |
dc.relation.ispartofpageto | 3362 | |
dc.relation.ispartofissue | 8 | |
dc.relation.ispartofjournal | Journal of Clinical Investigation | |
dc.relation.ispartofvolume | 123 | |
dc.rights.retention | Y | |
dc.subject.fieldofresearch | Infectious agents | |
dc.subject.fieldofresearch | Biomedical and clinical sciences | |
dc.subject.fieldofresearch | Cellular immunology | |
dc.subject.fieldofresearch | Biological sciences | |
dc.subject.fieldofresearch | Health sciences | |
dc.subject.fieldofresearchcode | 310702 | |
dc.subject.fieldofresearchcode | 32 | |
dc.subject.fieldofresearchcode | 320404 | |
dc.subject.fieldofresearchcode | 31 | |
dc.subject.fieldofresearchcode | 42 | |
dc.title | Cross-species malaria immunity induced by chemically attenuated parasites | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.faculty | Office of the Snr Dep Vice Chancellor, Institute for Glycomics | |
gro.rights.copyright | © 2013 American Society for Clinical Investigation (ASCI). The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version. | |
gro.date.issued | 2013 | |
gro.hasfulltext | Full Text | |
gro.griffith.author | Good, Michael F. | |
gro.griffith.author | El-Deeb, Ibrahim Mustafa | |
gro.griffith.author | Stanisic, Danielle | |