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  • Structural insights into the histone H1-nucleosome complex.

    Author(s)
    Zhou, Bing-Rui
    Feng, Hanqiao
    Kato, Hidenori
    Dai, Liang
    Yang, Yuedong
    Zhou, Yaoqi
    Bai, Yawen
    Griffith University Author(s)
    Zhou, Yaoqi
    Yang, Yuedong
    Year published
    2013
    Metadata
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    Abstract
    Linker H1 histones facilitate formation of higher-order chromatin structures and play important roles in various cell functions. Despite several decades of effort, the structural basis of how H1 interacts with the nucleosome remains elusive. Here, we investigated Drosophila H1 in complex with the nucleosome, using solution nuclear magnetic resonance spectroscopy and other biophysical methods. We found that the globular domain of H1 bridges the nucleosome core and one 10-base pair linker DNA asymmetrically, with its a3 helix facing the nucleosomal DNA near the dyad axis. Two short regions in the C-terminal tail of H1 and the ...
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    Linker H1 histones facilitate formation of higher-order chromatin structures and play important roles in various cell functions. Despite several decades of effort, the structural basis of how H1 interacts with the nucleosome remains elusive. Here, we investigated Drosophila H1 in complex with the nucleosome, using solution nuclear magnetic resonance spectroscopy and other biophysical methods. We found that the globular domain of H1 bridges the nucleosome core and one 10-base pair linker DNA asymmetrically, with its a3 helix facing the nucleosomal DNA near the dyad axis. Two short regions in the C-terminal tail of H1 and the C-terminal tail of one of the two H2A histones are also involved in the formation of the H1-nucleosome complex. Our results lead to a residue-specific structural model for the globular domain of the Drosophila H1 in complex with the nucleosome, which is different from all previous experiment-based models and has implications for chromatin dynamics in vivo.
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    Journal Title
    Proceedings of the National Academy of Sciences of the United States of America
    Volume
    110
    Issue
    48
    DOI
    https://doi.org/10.1073/pnas.1314905110
    Subject
    Structural biology (incl. macromolecular modelling)
    Publication URI
    http://hdl.handle.net/10072/56937
    Collection
    • Journal articles

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