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dc.contributor.authorDay, Christopher J
dc.contributor.authorTram, Greg
dc.contributor.authorHartley-Tassell, Lauren E
dc.contributor.authorTiralongo, Joe
dc.contributor.authorKorolik, Victoria
dc.date.accessioned2017-05-03T11:42:36Z
dc.date.available2017-05-03T11:42:36Z
dc.date.issued2013
dc.date.modified2014-02-20T22:23:35Z
dc.identifier.issn1471-2180
dc.identifier.doi10.1186/1471-2180-13-228
dc.identifier.urihttp://hdl.handle.net/10072/56956
dc.description.abstractBackground Campylobacter jejuni strain 11168 was demonstrated to have a broad specificity for eukaryotic surface glycosylation using glycan array analysis. The initial screen indicated that sialic acid and mannose are important binding partners after environmental stress, while galactose and fucose structures are likely to be involved in persistent infection. Results In this broader study, five additional human/clinical isolates and six chicken isolates were fully assessed to determine their glycan binding capacity using an extended glycan array. C. jejuni 11168 was rescreened here due to the presence of glycoaminoglycan (GAG) and other structures that were not available on our previous glycan array. The current array analysis of additional C. jejuni strains confirmed the growth condition dependent differences in glycan binding that was previously observed for C. jejuni 11168. We noted strain to strain variations, particularly for the human isolates C. jejuni 520 and 81116 and the chicken isolate C. jejuni 331, with the majority of differences observed in galactose, mannose and GAG binding. Chicken isolates were found to bind to a broader range of glycans compared to the human isolates, recognising branched mannose and carageenan (red seaweed) glycans. Glycan array data was confirmed using cell-based lectin inhibition assays with the fucose (UEA-I) and mannose (ConA) binding lectins. Conclusions This study confirms that all C. jejuni strains tested bind to a broad range of glycans, with the majority of strains (all except 81116) altering recognition of sialic acid and mannose after environmental stress. Galactose and fucose structures were bound best by all strains when C. jejuni was grown under host like conditions confirming the likelihood of these structures being involved in persistent infection.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent320166 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherBioMed Central
dc.publisher.placeUnited Kingdom
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom228
dc.relation.ispartofpageto240
dc.relation.ispartofjournalBMC Microbiology
dc.relation.ispartofvolume13
dc.rights.retentionY
dc.subject.fieldofresearchBiological sciences
dc.subject.fieldofresearchBacteriology
dc.subject.fieldofresearchAgricultural, veterinary and food sciences
dc.subject.fieldofresearchBiomedical and clinical sciences
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchMedical microbiology
dc.subject.fieldofresearchcode31
dc.subject.fieldofresearchcode310701
dc.subject.fieldofresearchcode30
dc.subject.fieldofresearchcode32
dc.subject.fieldofresearchcode3107
dc.subject.fieldofresearchcode3207
dc.titleAssessment of glycan interactions of clinical and avian isolates of Campylobacter jejuni
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by/2.0
gro.description.notepublicPage numbers are not for citation purposes. Instead, this article has the unique article number of 228.
gro.rights.copyright© 2013 Day et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.date.issued2013
gro.hasfulltextFull Text
gro.griffith.authorKorolik, Victoria
gro.griffith.authorDay, Christopher J.
gro.griffith.authorTiralongo, Joe
gro.griffith.authorTram, Greg C.


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