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dc.contributor.authorCosta, Pedro F
dc.contributor.authorVaquette, Cedryck
dc.contributor.authorZhang, Qiyi
dc.contributor.authorReis, Rui L
dc.contributor.authorIvanovski, Saso
dc.contributor.authorHutmacher, Dietmar W
dc.date.accessioned2017-05-03T16:05:36Z
dc.date.available2017-05-03T16:05:36Z
dc.date.issued2014
dc.date.modified2014-03-27T04:29:54Z
dc.identifier.issn0303-6979
dc.identifier.doi10.1111/jcpe.12214
dc.identifier.urihttp://hdl.handle.net/10072/57372
dc.description.abstractAim This study investigated the ability of an osteoconductive biphasic scaffold to simultaneously regenerate alveolar bone, periodontal ligament and cementum. Materials and Methods A biphasic scaffold was built by attaching a fused deposition modelled bone compartment to a melt electrospun periodontal compartment. The bone compartment was coated with a calcium phosphate (CaP) layer for increasing osteoconductivity, seeded with osteoblasts and cultured in vitro for 6 weeks. The resulting constructs were then complemented with the placement of PDL cell sheets on the periodontal compartment, attached to a dentin block and subcutaneously implanted into athymic rats for 8 weeks. Scanning electron microscopy, X-ray diffraction, alkaline phosphatase and DNA content quantification, confocal laser microscopy, micro computerized tomography and histological analysis were employed to evaluate the scaffold's performance. Results The in vitro study showed that alkaline phosphatase activity was significantly increased in the CaP-coated samples and they also displayed enhanced mineralization. In the in vivo study, significantly more bone formation was observed in the coated scaffolds. Histological analysis revealed that the large pore size of the periodontal compartment permitted vascularization of the cell sheets, and periodontal attachment was achieved at the dentin interface. Conclusions This work demonstrates that the combination of cell sheet technology together with an osteoconductive biphasic scaffold could be utilized to address the limitations of current periodontal regeneration techniques.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom283
dc.relation.ispartofpageto294
dc.relation.ispartofissue3
dc.relation.ispartofjournalJournal of Clinical Periodontology
dc.relation.ispartofvolume41
dc.rights.retentionY
dc.subject.fieldofresearchDentistry
dc.subject.fieldofresearchPeriodontics
dc.subject.fieldofresearchcode3203
dc.subject.fieldofresearchcode320310
dc.titleAdvanced tissue engineering scaffold design for regeneration of the complex hierarchical periodontal structure
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2013
gro.hasfulltextNo Full Text
gro.griffith.authorIvanovski, Saso
gro.griffith.authorVaquette, Cedryck
gro.griffith.authorHutmacher, Dietmar W.


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