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dc.contributor.authorBianco, JI
dc.contributor.authorPerry, C
dc.contributor.authorHarkin, DG
dc.contributor.authorMackay-Sim, A
dc.contributor.authorFeron, F
dc.date.accessioned2017-05-03T11:32:25Z
dc.date.available2017-05-03T11:32:25Z
dc.date.issued2004
dc.date.modified2009-09-21T05:50:44Z
dc.identifier.issn0894-1491
dc.identifier.doi10.1002/glia.10298
dc.identifier.urihttp://hdl.handle.net/10072/5752
dc.description.abstractSeveral studies have demonstrated the potential of olfactory ensheathing cells for the repair of central and peripheral nerve injury. However, the majority of these studies have been performed with olfactory ensheathing cells derived from the olfactory bulbs, situated inside the skull. A more clinically relevant source of olfactory ensheathing cells is the olfactory mucosa, located in the nose. To be successful, an autologous transplant of nasal ensheathing glia would require a large number of purified cells. To address this issue, we have focused our research on three neurotrophic factors, namely nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and neurotrophin 3 (NT3). We show here that their respective receptors, TrkA, TrkB, TrkC, as well as p75NTR (the low affinity NGF receptor), are expressed in vitro by the nasal ensheathing cells; the three neurotrophins promote purification and proliferation of these glial cells, with an optimal concentration of 50 ng/ml; and human ensheathing cells can be easily biopsied and highly purified using a serum-free medium supplemented with NT3. This technique opens the door for clinical trials in which nasal ensheathing cells will be autotransplanted in humans suffering from nerve injury.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherJohn Wiley
dc.publisher.placeBognor regis, UK
dc.publisher.urihttp://www3.interscience.wiley.com/journal/37090/home
dc.relation.ispartofpagefrom111
dc.relation.ispartofpageto123
dc.relation.ispartofjournalGlia
dc.relation.ispartofvolume45
dc.subject.fieldofresearchNeurosciences
dc.subject.fieldofresearchcode1109
dc.titleNeurotrophin 3 promotes purification and proliferation of olfactory ensheathing cells from human nose.
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyright© 2004 John Wiley & Sons, Ltd. Self-archiving of the author-manuscript version is not yet supported by this publisher. Please refer to the journal link for access to the definitive, published version or contact the author for more information.
gro.date.issued2004
gro.hasfulltextNo Full Text
gro.griffith.authorMackay-Sim, Alan
gro.griffith.authorBianco, John I.
gro.griffith.authorFeron, Francois
gro.griffith.authorHarkin, Damien G.


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