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dc.contributor.authorWoolfit, Megan
dc.contributor.authorIturbe-Ormaetxe, Inaki
dc.contributor.authorBrownlie, Jeremy C
dc.contributor.authorWalker, Thomas
dc.contributor.authorRiegler, Markus
dc.contributor.authorSeleznev, Andrei
dc.contributor.authorPopovici, Jean
dc.contributor.authorRances, Edwige
dc.contributor.authorWee, Bryan A
dc.contributor.authorPavlides, Jennifer
dc.contributor.authorSullivan, Mitchell J
dc.contributor.authorBeatson, Scott A
dc.contributor.authorLane, Amanda
dc.contributor.authorSidhu, Manpreet
dc.contributor.authorMcMeniman, Conor J
dc.contributor.authorMcGraw, Elizabeth A
dc.contributor.authorO'Neill, Scott L
dc.date.accessioned2017-05-03T15:30:54Z
dc.date.available2017-05-03T15:30:54Z
dc.date.issued2013
dc.date.modified2014-04-10T05:22:17Z
dc.identifier.issn1759-6653
dc.identifier.doi10.1093/gbe/evt169
dc.identifier.urihttp://hdl.handle.net/10072/58215
dc.description.abstractMost strains of the widespread endosymbiotic bacterium Wolbachia pipientis are benign or behave as reproductive parasites. The pathogenic strain wMelPop is a striking exception, however: it overreplicates in its insect hosts and causes severe life shortening. The mechanism of this pathogenesis is currently unknown. We have sequenced the genomes of three variants of wMelPop and of the closely related nonpathogenic strain wMelCS. We show that the genomes of wMelCS and wMelPop appear to be identical in the nonrepeat regions of the genome and differ detectably only by the triplication of a 19-kb region that is unlikely to be associated with life shortening, demonstrating that dramatic differences in the host phenotype caused by this endosymbiont may be the result of only minor genetic changes. We also compare the genomes of the original wMelPop strain from Drosophila melanogaster and two sequential derivatives, wMelPop-CLA and wMelPop-PGYP. To develop wMelPop as a novel biocontrol agent, it was first transinfected into and passaged in mosquito cell lines for approximately 3.5 years, generating wMelPop-CLA. This cell line-passaged strain was then transinfected into Aedes aegypti mosquitoes, creating wMelPop-PGYP, which was sequenced after 4 years in the insect host. We observe a rapid burst of genomic changes during cell line passaging, but no further mutations were detected after transinfection into mosquitoes, indicating either that host preadaptation had occurred in cell lines, that cell lines are a more selectively permissive environment than animal hosts, or both. Our results provide valuable data on the rates of genomic and phenotypic change in Wolbachia associated with host shifts over short time scales.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent1312271 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherOxford University Press
dc.publisher.placeUnited Kingdom
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom2189
dc.relation.ispartofpageto2204
dc.relation.ispartofissue11
dc.relation.ispartofjournalGenome Biology and Evolution
dc.relation.ispartofvolume5
dc.rights.retentionY
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchEvolutionary biology
dc.subject.fieldofresearchBiological adaptation
dc.subject.fieldofresearchHost-parasite interactions
dc.subject.fieldofresearchGenetics
dc.subject.fieldofresearchGenomics
dc.subject.fieldofresearchcode3101
dc.subject.fieldofresearchcode3104
dc.subject.fieldofresearchcode310403
dc.subject.fieldofresearchcode310407
dc.subject.fieldofresearchcode3105
dc.subject.fieldofresearchcode310509
dc.titleGenomic Evolution of the Pathogenic Wolbachia Strain, wMelPop
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by/3.0/
gro.rights.copyright© 2013 The Author(s). Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.date.issued2013
gro.hasfulltextFull Text
gro.griffith.authorBrownlie, Jeremy


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