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dc.contributor.authorLi, R.
dc.contributor.authorCarmichael, W.W.
dc.contributor.authorBrittain, S.
dc.contributor.authorEaglesham, G.K.
dc.contributor.authorShaw, Glendon Reginald
dc.contributor.authorMahakhant, A.
dc.contributor.authorNoparatnaraporn, N.
dc.contributor.authorYongmanitchai, W.
dc.contributor.authorKaya, K.
dc.contributor.authorWatanabe, M.M.
dc.date.accessioned2006-07-20
dc.date.accessioned2014-04-14T23:26:04Z
dc.date.accessioned2017-03-02T00:01:10Z
dc.date.available2017-03-02T00:01:10Z
dc.date.issued2001
dc.date.modified2014-04-14T23:26:04Z
dc.identifier.issn0041-0101
dc.identifier.doi10.1016/S0041-0101(00)00236-1
dc.identifier.urihttp://hdl.handle.net/10072/58361
dc.description.abstractA strain of Cylindrospermopsis (Cyanobacteria) isolated from a fishpond in Thailand was examined for its taxonomy based upon morphology and 16S rRNA gene sequence. It was also examined for production of the hepatotoxic cyanotoxin called cylindrospermopsin (CYN) and deoxycylindrospermopsin (deoxy-CYN). The strain (CY-Thai) was identified as C. raciborskii (Woloszynska) Seenaya and Subba Raju based upon morphological examination which was confirmed by 16S rRNA gene sequences and phylogenetic comparisons based upon its 16S rRNA gene. The alkaloid heptatotoxin CYN was confirmed using mouse bioassay, HPLC and HPLC-MS/MS while deoxy-CYN was confirmed using HPLC-MS/MS. The mouse bioassay gave a minimum lethal dose at 250 mg dry weight cells/kg body weight within 24 h and 125 mg/kg at 72 h, with signs of poisoning the same as in literature reports for CYN. HPLC chromatographic comparison of the CY-Thai toxin with standard CYN gave the same retention time and an absorbance maximum at 262 nm. HPLC-MS/MS confirmed the presence of CYN (M+H 416) and deoxy-CYN (M+H 400). The CYN content in strain CY-Thai was estimated at 1.02 mg/g and approximately 1/10 of this amount for deoxy-CYN. This is the first report from Asia of a CYN, deoxy-CYN producing Cylindrospermopsis raciborskii.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.publisherElsevier Science
dc.publisher.placeAmsterdam
dc.relation.ispartofpagefrom973
dc.relation.ispartofpageto980
dc.relation.ispartofjournalToxicon
dc.relation.ispartofvolume39
dc.subject.fieldofresearchPharmacology and Pharmaceutical Sciences
dc.subject.fieldofresearchcode1115
dc.titleIsolation and identification of the cyanotoxin cylindrospermopsin and deoxy-cylindrospermopsin from a Thailand strain of Cylindrospermopsis raciborskii (Cyanobacteria).
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codec1x
gro.facultyGriffith Health Faculty
gro.date.issued2001
gro.hasfulltextNo Full Text
gro.griffith.authorShaw, Glendon R.


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