Association of antigens to ISCOMATRIX adjuvant using metal chelation leads to improved CTL responses
Author(s)
Stewart, Trina
Griffith University Author(s)
Year published
2004
Metadata
Show full item recordAbstract
The association of antigen with ISCOMATRIXTM adjuvant has been shown to be important for the optimal induction of cytotoxic T lymphocyte (CTL) responses. Here, we describe a simple broadly applicable method for associating recombinant proteins with hexahistidine tags toISCOMATRIXTM adjuvant utilising metal-affinity chelating interactions. The metal chelation binding step can be performed in a wide range of buffers, including commonly used denaturants such as urea, which makes it an ideal strategy for formulating proteins which are otherwise insoluble. Following association of protein with the chelating ISCOMATRIXTM adjuvant, ...
View more >The association of antigen with ISCOMATRIXTM adjuvant has been shown to be important for the optimal induction of cytotoxic T lymphocyte (CTL) responses. Here, we describe a simple broadly applicable method for associating recombinant proteins with hexahistidine tags toISCOMATRIXTM adjuvant utilising metal-affinity chelating interactions. The metal chelation binding step can be performed in a wide range of buffers, including commonly used denaturants such as urea, which makes it an ideal strategy for formulating proteins which are otherwise insoluble. Following association of protein with the chelating ISCOMATRIXTM adjuvant, the denaturant can be removed. Further, we show enhanced CTL responses with a protein-associated chelating ISCOMATRIXTM vaccine compared to a non-associated ISCOMATRIXTM vaccine.
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View more >The association of antigen with ISCOMATRIXTM adjuvant has been shown to be important for the optimal induction of cytotoxic T lymphocyte (CTL) responses. Here, we describe a simple broadly applicable method for associating recombinant proteins with hexahistidine tags toISCOMATRIXTM adjuvant utilising metal-affinity chelating interactions. The metal chelation binding step can be performed in a wide range of buffers, including commonly used denaturants such as urea, which makes it an ideal strategy for formulating proteins which are otherwise insoluble. Following association of protein with the chelating ISCOMATRIXTM adjuvant, the denaturant can be removed. Further, we show enhanced CTL responses with a protein-associated chelating ISCOMATRIXTM vaccine compared to a non-associated ISCOMATRIXTM vaccine.
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Journal Title
Vaccine
Volume
22
Subject
Applied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies)
Biological Sciences
Agricultural and Veterinary Sciences
Medical and Health Sciences