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  • Association of antigens to ISCOMATRIX adjuvant using metal chelation leads to improved CTL responses

    Author(s)
    Stewart, Trina
    Griffith University Author(s)
    Stewart, Trina
    Year published
    2004
    Metadata
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    Abstract
    The association of antigen with ISCOMATRIXTM adjuvant has been shown to be important for the optimal induction of cytotoxic T lymphocyte (CTL) responses. Here, we describe a simple broadly applicable method for associating recombinant proteins with hexahistidine tags toISCOMATRIXTM adjuvant utilising metal-affinity chelating interactions. The metal chelation binding step can be performed in a wide range of buffers, including commonly used denaturants such as urea, which makes it an ideal strategy for formulating proteins which are otherwise insoluble. Following association of protein with the chelating ISCOMATRIXTM adjuvant, ...
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    The association of antigen with ISCOMATRIXTM adjuvant has been shown to be important for the optimal induction of cytotoxic T lymphocyte (CTL) responses. Here, we describe a simple broadly applicable method for associating recombinant proteins with hexahistidine tags toISCOMATRIXTM adjuvant utilising metal-affinity chelating interactions. The metal chelation binding step can be performed in a wide range of buffers, including commonly used denaturants such as urea, which makes it an ideal strategy for formulating proteins which are otherwise insoluble. Following association of protein with the chelating ISCOMATRIXTM adjuvant, the denaturant can be removed. Further, we show enhanced CTL responses with a protein-associated chelating ISCOMATRIXTM vaccine compared to a non-associated ISCOMATRIXTM vaccine.
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    Journal Title
    Vaccine
    Volume
    22
    DOI
    https://doi.org/10.1016/j.vaccine.2004.03.054
    Subject
    Applied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies)
    Biological Sciences
    Agricultural and Veterinary Sciences
    Medical and Health Sciences
    Publication URI
    http://hdl.handle.net/10072/58405
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    • Journal articles

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