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  • Gender-specific transcriptional profiling of marine medaka (Oryzias melastigma) liver upon BDE-47 exposure

    Author(s)
    Yu, WK
    Shi, YF
    Fong, CC
    Chen, Y
    van de Merwe, JP
    Chan, Alice KY
    Wei, F
    Bo, J
    Ye, R
    Au, Doris WT
    Wu, Rudolf SS
    Yang, MS
    Griffith University Author(s)
    van de Merwe, Jason P.
    Year published
    2013
    Metadata
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    Abstract
    Marine medaka (Oryzias melastigma) were exposed to 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) to investigate the gender-specific transcriptional profiles of liver tissue in response to this flame retardant. A cDNA library of O. melastigma was constructed, and 2304 clones were amplified from the library to fabricate a cDNA microarray. Sequences of these genes were assembled into 1800 sequences using Geneious, a bioinformatics software. Corresponding expressed sequence tags were blasted against the National Centre for Biotechnology Information non-redundant database and further classified into various biological categories ...
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    Marine medaka (Oryzias melastigma) were exposed to 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) to investigate the gender-specific transcriptional profiles of liver tissue in response to this flame retardant. A cDNA library of O. melastigma was constructed, and 2304 clones were amplified from the library to fabricate a cDNA microarray. Sequences of these genes were assembled into 1800 sequences using Geneious, a bioinformatics software. Corresponding expressed sequence tags were blasted against the National Centre for Biotechnology Information non-redundant database and further classified into various biological categories according to the Gene Ontology project. Male and female three-month-old were fed a diet of BDE-47 contaminated Artemia at low dosage (290.3 ᠱ72.3 ng BDE-47/day) and high dosage (580.5 ᠳ44.6 ng BDE-47/day) for 5 and 21 days, respectively. The transcriptional profiles of O. melastigma liver were then generated by the species-specific cDNA microrarray. The results from microarray analysis suggested very different gene expression patterns between males and females for both BDE-47 exposure-dose and exposure-time, with male livers having stronger gene regulatory responses than female livers. Importantly, our results revealed that in male O. melastigma only, BDE-47 exposure may activate phosphoinositide-3-kinase and mitogen-activated protein kinase, proteins that play importance roles in cell growth, proliferation and survival.
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    Journal Title
    Comparative Biochemistry and Physiology, Part D
    Volume
    8
    Issue
    3
    DOI
    https://doi.org/10.1016/j.cbd.2013.06.004
    Subject
    Genetics
    Animal cell and molecular biology
    Publication URI
    http://hdl.handle.net/10072/58488
    Collection
    • Journal articles

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