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dc.contributor.authorGoldstone, S
dc.contributor.authorPavey, S
dc.contributor.authorForrest, A
dc.contributor.authorSinnamon, J
dc.contributor.authorGabrielli, B
dc.date.accessioned2007-12-05
dc.date.accessioned2014-05-15T22:03:11Z
dc.date.accessioned2017-03-01T23:51:22Z
dc.date.available2017-03-01T23:51:22Z
dc.date.issued2001
dc.date.modified2014-05-15T22:03:11Z
dc.identifier.issn0950-9232
dc.identifier.urihttp://hdl.handle.net/10072/59134
dc.description.abstractCyclin A/cdk2 is active during S and G2 phases of the cell cycle, but its regulation and function during G2 phase is poorly understood. In this study we have examined the regulation of cyclin A/cdk2 activity during normal G2 phase progression and in genotoxin-induced G2 arrest. We show that cyclin A/cdk2 is activated in early G2 phase by a cdc25 activity. In the G2 phase checkpoint arrest initiated in response to various forms of DNA damage, the cdc25-dependent activation of both cyclin A/cdk2 and cyclin B1/cdc2 is blocked. Ectopic expression of cdc25B, but not cdc25C, in G2 phase arrested cells efficiently activated both cyclin A/cdk2 and cyclin B1/cdc2. Finally, we demonstrate that the block in cyclin A/cdk2 activation in the G2 checkpoint arrest is independent of ATM/ATR. We speculate that the ATM/ATR-independent block in G2 phase cyclin A/cdk2 activation may act as a further layer of checkpoint control, and that blocking G2 phase cyclin A/cdk2 activation contributes to the G2 phase checkpoint arrest.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherNature Publishing Group
dc.publisher.placeUnited Kingdom
dc.publisher.urihttp://www.nature.com/onc/journal/v20/n8/abs/1204177a.html
dc.relation.ispartofpagefrom921
dc.relation.ispartofpageto932
dc.relation.ispartofissue8
dc.relation.ispartofjournalOncogene
dc.relation.ispartofvolume20
dc.subject.fieldofresearchClinical Sciences
dc.subject.fieldofresearchOncology and Carcinogenesis
dc.subject.fieldofresearchcode1103
dc.subject.fieldofresearchcode1112
dc.titleCdc25-dependent activation of cyclin A/cdk2 is blocked in G2 phase arrested cells independently of ATM/ATR
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codec1x
gro.facultyEskitis, Inst Cell&Molecular Therapies
gro.date.issued2001
gro.hasfulltextNo Full Text
gro.griffith.authorGabrielli, Brian
gro.griffith.authorForrest, Alistair RR.


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