dc.contributor.author | Yu, Hai | |
dc.contributor.author | Chokhawala, Harshal A. | |
dc.contributor.author | Huang, Shengshu | |
dc.contributor.author | Chen, Xi | |
dc.date.accessioned | 2017-05-03T15:55:12Z | |
dc.date.available | 2017-05-03T15:55:12Z | |
dc.date.issued | 2006 | |
dc.date.modified | 2014-05-15T22:03:42Z | |
dc.identifier.issn | 17542189 | |
dc.identifier.doi | 10.1038/nprot.2006.401 | |
dc.identifier.uri | http://hdl.handle.net/10072/59135 | |
dc.description.abstract | Chemoenzymatic synthesis, which combines the flexibility of chemical synthesis and the high selectivity of enzymatic synthesis, is a powerful approach to obtain complex carbohydrates. It is a preferred method for synthesizing sialic acid-containing structures, including those with diverse naturally occurring and non-natural sialic acid forms, different sialyl linkages and different glycans that link to the sialic acid. Starting from N-acetylmannosamine, mannose or their chemically or enzymatically modified derivatives, sialic acid aldolase-catalyzed condensation reaction leads to the formation of sialic acids and their derivatives. These compounds are subsequently activated by a CMP-sialic acid synthetase and transferred to a wide range of suitable acceptors by a suitable sialyltransferase for the formation of sialosides containing natural and non-natural functionalities. The three-enzyme coupled synthesis of sialosides can be carried out in one pot without the isolation of intermediates. The time for synthesis is 4-18 h. Purification and characterization of the product can be completed within 2-3 d. | |
dc.description.peerreviewed | Yes | |
dc.description.publicationstatus | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Nature Publishing Group | |
dc.publisher.place | United Kingdom | |
dc.relation.ispartofstudentpublication | N | |
dc.relation.ispartofpagefrom | 2485 | |
dc.relation.ispartofpageto | 2492 | |
dc.relation.ispartofissue | 5 | |
dc.relation.ispartofjournal | Nature Protocols | |
dc.relation.ispartofvolume | 1 | |
dc.rights.retention | Y | |
dc.subject.fieldofresearch | Organic Chemical Synthesis | |
dc.subject.fieldofresearch | Chemical Sciences | |
dc.subject.fieldofresearch | Biological Sciences | |
dc.subject.fieldofresearch | Medical and Health Sciences | |
dc.subject.fieldofresearchcode | 030503 | |
dc.subject.fieldofresearchcode | 03 | |
dc.subject.fieldofresearchcode | 06 | |
dc.subject.fieldofresearchcode | 11 | |
dc.title | One-pot three-enzyme chemoenzymatic approach to the synthesis of sialosides containing natural and non-natural functionalities | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.date.issued | 2006 | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Huang, Shengshu | |