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dc.contributor.authorYu, Hai
dc.contributor.authorChokhawala, Harshal A.
dc.contributor.authorHuang, Shengshu
dc.contributor.authorChen, Xi
dc.date.accessioned2017-05-03T15:55:12Z
dc.date.available2017-05-03T15:55:12Z
dc.date.issued2006
dc.date.modified2014-05-15T22:03:42Z
dc.identifier.issn17542189
dc.identifier.doi10.1038/nprot.2006.401
dc.identifier.urihttp://hdl.handle.net/10072/59135
dc.description.abstractChemoenzymatic synthesis, which combines the flexibility of chemical synthesis and the high selectivity of enzymatic synthesis, is a powerful approach to obtain complex carbohydrates. It is a preferred method for synthesizing sialic acid-containing structures, including those with diverse naturally occurring and non-natural sialic acid forms, different sialyl linkages and different glycans that link to the sialic acid. Starting from N-acetylmannosamine, mannose or their chemically or enzymatically modified derivatives, sialic acid aldolase-catalyzed condensation reaction leads to the formation of sialic acids and their derivatives. These compounds are subsequently activated by a CMP-sialic acid synthetase and transferred to a wide range of suitable acceptors by a suitable sialyltransferase for the formation of sialosides containing natural and non-natural functionalities. The three-enzyme coupled synthesis of sialosides can be carried out in one pot without the isolation of intermediates. The time for synthesis is 4-18 h. Purification and characterization of the product can be completed within 2-3 d.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherNature Publishing Group
dc.publisher.placeUnited Kingdom
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom2485
dc.relation.ispartofpageto2492
dc.relation.ispartofissue5
dc.relation.ispartofjournalNature Protocols
dc.relation.ispartofvolume1
dc.rights.retentionY
dc.subject.fieldofresearchOrganic Chemical Synthesis
dc.subject.fieldofresearchChemical Sciences
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchMedical and Health Sciences
dc.subject.fieldofresearchcode030503
dc.subject.fieldofresearchcode03
dc.subject.fieldofresearchcode06
dc.subject.fieldofresearchcode11
dc.titleOne-pot three-enzyme chemoenzymatic approach to the synthesis of sialosides containing natural and non-natural functionalities
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2006
gro.hasfulltextNo Full Text
gro.griffith.authorHuang, Shengshu


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