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  • Monocyte chemoattractant protein-1 is a mediator of the anabolic action of parathyroid hormone on bone

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    Author(s)
    Tamasi, Joseph A
    Vasilov, Anatoliy
    Shimizu, Emi
    Benton, Noah
    Johnson, Joshua
    Bitel, Claudine L
    Morrison, Nigel
    Partridge, Nicola C
    Griffith University Author(s)
    Morrison, Nigel A.
    Year published
    2013
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    Abstract
    Parathyroid hormone (PTH) has a significant role as an anabolic hormone in bone when administered by intermittent injection. Previous microarray studies in our laboratory have shown that the most highly regulated gene, monocyte chemoattractant protein-1 (MCP-1), is rapidly and transiently induced when hPTH(1-34) is injected intermittently in rats. Through further in vivo studies, we found that rats treated with hPTH(1-34) showed a significant increase in serum MCP-1 levels 2 hours after PTH injection compared to basal levels. Using immunohistochemistry, increased MCP-1 expression in osteoblasts and osteocytes is evident after ...
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    Parathyroid hormone (PTH) has a significant role as an anabolic hormone in bone when administered by intermittent injection. Previous microarray studies in our laboratory have shown that the most highly regulated gene, monocyte chemoattractant protein-1 (MCP-1), is rapidly and transiently induced when hPTH(1-34) is injected intermittently in rats. Through further in vivo studies, we found that rats treated with hPTH(1-34) showed a significant increase in serum MCP-1 levels 2 hours after PTH injection compared to basal levels. Using immunohistochemistry, increased MCP-1 expression in osteoblasts and osteocytes is evident after PTH treatment. PTH also increased the number of marrow macrophages. MCP-1 knockout mice injected daily with hPTH(1-34) showed less trabecular bone mineral density and bone volume compared to wild type mice as measured by pQCT and microCT. Histomorphometric analysis revealed that the increase in osteoclast surface and osteoclast number observed with intermittent PTH treatment in the wild type mice was completely eliminated in the MCP-1 null mice, as well as much lower numbers of macrophages. Consequently, the lack of osteoclast and macrophage activity in the MCP-1 null mice was paralleled by a reduction in bone formation. We conclude that osteoblast and osteocyte MCP-1 expression is an important mediator for the anabolic effects of PTH on bone.
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    Journal Title
    Journal of Bone and Mineral Research
    Volume
    28
    Issue
    9
    DOI
    https://doi.org/10.1002/jbmr.1933
    Copyright Statement
    © 2013 American Society for Bone and Mineral Research. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
    Subject
    Biological sciences
    Engineering
    Biomedical and clinical sciences
    Publication URI
    http://hdl.handle.net/10072/59206
    Collection
    • Journal articles

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