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dc.contributor.authorWarren, Hilary S
dc.contributor.authorWu, Fan
dc.contributor.authorHorn, Peggy L
dc.contributor.authorPyne, David B
dc.contributor.authorWest, Nicholas P
dc.contributor.authorCripps, Allan W
dc.date.accessioned2017-05-03T14:31:24Z
dc.date.available2017-05-03T14:31:24Z
dc.date.issued2013
dc.date.modified2014-05-22T22:15:30Z
dc.identifier.issn0022-1759
dc.identifier.doi10.1016/j.jim.2013.03.010
dc.identifier.urihttp://hdl.handle.net/10072/59207
dc.description.abstractIn this technical note we provide data useful for the clinical application of the target-induced Natural Killer (NK) loss (TINKL) assay. The TINKL assay is a sensitive flow cytometry-based assay for measuring NK cell function. Loss of NK cells from the lymphocyte gate occurs following culture with K562 (the prototypic target cell for natural killing) and antibody-coated target cells (for antibody-dependent killing). By analysis of multiple samples of PBMC from single donors we document the intra-experimental variability and the inter-experimental variability of the assay. The intra-experimental coefficient of variation (CV) was on average 11% for natural killing and 3% for antibody-dependent killing, compared to 14% and 9% respectively for the inter-experimental variation. Analysis of a 123 normal healthy adults shows large variability in the functional capacity of NK cells in the population both for natural killing (CV 33%) and antibody-dependent killing (CV 27%).
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.publisher.placeNetherlands
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom68
dc.relation.ispartofpageto70
dc.relation.ispartofissue1-2
dc.relation.ispartofjournalJournal of Immunological Methods
dc.relation.ispartofvolume392
dc.rights.retentionY
dc.subject.fieldofresearchOther biological sciences not elsewhere classified
dc.subject.fieldofresearchImmunology
dc.subject.fieldofresearchMedical microbiology
dc.subject.fieldofresearchOphthalmology and optometry
dc.subject.fieldofresearchcode319999
dc.subject.fieldofresearchcode3204
dc.subject.fieldofresearchcode3207
dc.subject.fieldofresearchcode3212
dc.titlePeripheral blood natural killer (NK) cell function in healthy adults assessed using the target-induced NK loss (TINKL) assay
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorCripps, Allan W.
gro.griffith.authorWest, Nic P.


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