dc.contributor.author | M. Moyle, Peter | |
dc.contributor.author | Hari, Yashmeeta | |
dc.contributor.author | Huang, Ning | |
dc.contributor.author | Olive, Colleen | |
dc.contributor.author | F. Good, Michael | |
dc.contributor.author | Toth, Istvan | |
dc.date.accessioned | 2017-05-03T14:54:41Z | |
dc.date.available | 2017-05-03T14:54:41Z | |
dc.date.issued | 2007 | |
dc.date.modified | 2014-05-26T00:58:42Z | |
dc.identifier.issn | 00404039 | |
dc.identifier.doi | 10.1016/j.tetlet.2007.05.129 | |
dc.identifier.uri | http://hdl.handle.net/10072/59326 | |
dc.description.abstract | The synthesis of lipid core peptide (LCP) vaccines using stepwise solid-phase peptide synthesis commonly results in products which are difficult to purify to homogeneity. A new technique for synthesizing highly-pure, mono-epitopic, multi-valent LCP-systems using native chemical ligation is presented. Various conditions were assessed for ligating four copies of a thioester-modified 88/30 serotype group A streptococcal peptide antigen onto an LCP-system featuring four cysteine residues. Overall, the vaccine was synthesized in high purity (>99%), and high yield (90%) when the ligation reaction was performed in the presence of 1% sodium dodecyl sulfate and at elevated temperatures (37 é. | |
dc.description.peerreviewed | Yes | |
dc.description.publicationstatus | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Pergamon | |
dc.publisher.place | United Kingdom | |
dc.relation.ispartofstudentpublication | N | |
dc.relation.ispartofpagefrom | 4965 | |
dc.relation.ispartofpageto | 4967 | |
dc.relation.ispartofissue | 29 | |
dc.relation.ispartofjournal | Tetrahedron Letters | |
dc.relation.ispartofvolume | 48 | |
dc.rights.retention | Y | |
dc.subject.fieldofresearch | Organic Chemistry not elsewhere classified | |
dc.subject.fieldofresearch | Medicinal and Biomolecular Chemistry | |
dc.subject.fieldofresearch | Organic Chemistry | |
dc.subject.fieldofresearchcode | 030599 | |
dc.subject.fieldofresearchcode | 0304 | |
dc.subject.fieldofresearchcode | 0305 | |
dc.title | A technique for the synthesis of highly-pure, mono-epitopic, multi-valent lipid core peptide vaccines | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Good, Michael F. | |