An optics-based variable-temperature assay system for characterizing thermodynamics of biomolecular reactions on solid support
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A biological state is equilibrium of multiple concurrent biomolecular reactions. The relative importance of these reactions depends on physiological temperature typically between 10?àand 50?î Experimentally the temperature dependence of binding reaction constants reveals thermodynamics and thus details of these biomolecular processes. We developed a variable-temperature opto-fluidic system for real-time measurement of multiple (400-10?000) biomolecular binding reactions on solid supports from 10?àto 60?àwithin ᰮ1?î We illustrate the performance of this system with investigation of binding reactions of plant lectins (carbohydrate-binding proteins) with 24 synthetic glycans (i.e., carbohydrates). We found that the lectin-glycan reactions in general can be enthalpy-driven, entropy-driven, or both, and water molecules play critical roles in the thermodynamics of these reactions.
Review of Scientific Instruments
Chemical Sciences not elsewhere classified
Physical Sciences not elsewhere classified