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dc.contributor.authorBertolo, Alessandro
dc.contributor.authorMehr, Marco
dc.contributor.authorJanner-Jametti, Tiziana
dc.contributor.authorGraumann, Ursula
dc.contributor.authorAebli, Niklaus
dc.contributor.authorBaur, Martin
dc.contributor.authorFerguson, Stephen J
dc.contributor.authorStoyanov, Jivko V
dc.date.accessioned2018-02-14T12:30:29Z
dc.date.available2018-02-14T12:30:29Z
dc.date.issued2016
dc.date.modified2014-06-16T04:40:57Z
dc.identifier.issn1932-6254
dc.identifier.doi10.1002/term.1734
dc.identifier.urihttp://hdl.handle.net/10072/60274
dc.description.abstractHuman bone marrow-derived mesenchymal stem cells (MSCs) have limited growth potential in vitro and cease to divide due to replicative senescence, which from a tissue-engineering perspective has practical implications, such as defining the correct starting points for differentiation and transplantation. Time spent in culture before the loss of required differentiation potential is different and reflects patient variability, which is a problem for cell expansion. This study aimed to develop a score set which can be used to quantify the senescent state of MSCs and predict whether cells preserve their ability to differentiate to osteogenic, adipogenic and chondrogenic phenotypes, based on colony-forming unit (CFU) assay, population doubling time (PDT), senescence-associated ߭galactosidase (SA-߭Gal) activity, cell size, telomere length and gene expression of MSCs cultured in vitro over 11 passages. This set of morphological, physiological and genetic senescence markers was correlated to the ability of MSCs to differentiate. Differentiation efficiency was assessed by marker genes and protein expression. CFUs decreased with increasing passage number, whereas SA-߭Gal activity and PDT increased; however, the correlation with MSCs' differentiation potential was sometimes unexpected. The expression of genes related to senescence was higher in late-passage cells than in early-passage cells. Early-passage cells underwent efficient osteogenic differentiation, with mid-passage cells performing best in chondrogenic differentiation. Late-passage cells preserve only adipogenic differentiation potential. Based on this marker set, we propose a senescence score in which combined markers give a reliable quality control of MSCs, not depending only on mechanistic passage number.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherJossey Bass
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom149
dc.relation.ispartofpageto161
dc.relation.ispartofissue2
dc.relation.ispartofjournalJournal of Tissue Engineering and Regenerative Medicine
dc.relation.ispartofvolume10
dc.rights.retentionY
dc.subject.fieldofresearchBiomedical engineering
dc.subject.fieldofresearchBiomedical engineering not elsewhere classified
dc.subject.fieldofresearchClinical sciences
dc.subject.fieldofresearchClinical sciences not elsewhere classified
dc.subject.fieldofresearchMedical physiology
dc.subject.fieldofresearchMedical physiology not elsewhere classified
dc.subject.fieldofresearchcode4003
dc.subject.fieldofresearchcode400399
dc.subject.fieldofresearchcode3202
dc.subject.fieldofresearchcode320299
dc.subject.fieldofresearchcode3208
dc.subject.fieldofresearchcode320899
dc.titleAn in vitro expansion score for tissue-engineering applications with human bone marrow-derived mesenchymal stem cells
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorAebli, Nikolaus


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