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dc.contributor.authorWarnke, Patrick H
dc.contributor.authorAlamein, Mohammad
dc.contributor.authorSkabo, Stuart
dc.contributor.authorStephens, Sebastien
dc.contributor.authorBourke, Robert
dc.contributor.authorHeiner, Peter
dc.contributor.authorLiu, Qin
dc.date.accessioned2017-05-03T13:13:37Z
dc.date.available2017-05-03T13:13:37Z
dc.date.issued2013
dc.date.modified2014-06-16T04:42:18Z
dc.identifier.issn1742-7061
dc.identifier.doi10.1016/j.actbio.2013.07.029
dc.identifier.urihttp://hdl.handle.net/10072/60313
dc.description.abstractTransplanted retinal pigment epithelium (RPE) cells hold promise for treatment of age-related macular degeneration (AMD) and Stargardt disease (SD), but it is conceivable that the degenerated host Bruch's membrane (BM) as a natural substrate for RPE might not optimally support transplanted cell survival with correct cellular organization. We fabricated novel ultrathin three-dimensional (3-D) nanofibrous membranes from collagen type I and poly(lactic-co-glycolic acid) (PLGA) by an advanced clinical-grade needle-free electrospinning process. The nanofibrillar 3-D networks closely mimicked the fibrillar architecture of the native inner collagenous layer of human BM. Human RPE cells grown on our nanofibrous membranes bore a striking resemblance to native human RPE. They exhibited a correctly orientated monolayer with a polygonal cell shape and abundant sheet-like microvilli on their apical surfaces. RPE cells built tight junctions and expressed RPE65 protein. Flat 2-D PLGA film and cover glass as controls delivered inferior RPE layers. Our nanofibrous membranes may imitate the natural BM to such extent that they allow for the engineering of an in vivo-like human RPE monolayer that maintains the natural biofunctional characteristics. Such ultrathin membranes may provide a promising vehicle for a functional RPE cell monolayer implantation in the subretinal space in patients with AMD or SD.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.publisher.placeNetherlands
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom9414
dc.relation.ispartofpageto9422
dc.relation.ispartofissue12
dc.relation.ispartofjournalActa Biomaterialia
dc.relation.ispartofvolume9
dc.rights.retentionY
dc.subject.fieldofresearchDental Materials and Equipment
dc.subject.fieldofresearchOral and Maxillofacial Surgery
dc.subject.fieldofresearchcode110501
dc.subject.fieldofresearchcode110504
dc.titlePrimordium of an artificial Bruch's membrane made of nanofibers for engineering of retinal pigment epithelium cell monolayers
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Health, School of Dentistry and Oral Health
gro.hasfulltextNo Full Text
gro.griffith.authorStephens, Sebastien R.
gro.griffith.authorWarnke, Patrick H.
gro.griffith.authorAlamein, Mohammad


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