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dc.contributor.authorE. Hugo, Leonen_US
dc.contributor.authorMonkman, Jamesen_US
dc.contributor.authorA. Dave, Keyuren_US
dc.contributor.authorF. Wockner, Leesaen_US
dc.contributor.authorW. Birrell, Geoffen_US
dc.contributor.authorL. Norris, Emmaen_US
dc.contributor.authorJ. Kienzle, Vivianen_US
dc.contributor.authorSikulu, Maggyen_US
dc.contributor.authorRyan, Peter A.en_US
dc.contributor.authorJ. Gorman, Jefferyen_US
dc.contributor.authorH. Kay, Brianen_US
dc.date.accessioned2017-05-03T13:13:37Z
dc.date.available2017-05-03T13:13:37Z
dc.date.issued2013en_US
dc.date.modified2014-06-16T04:42:27Z
dc.identifier.issn19326203en_US
dc.identifier.doi10.1371/journal.pone.0058656en_US
dc.identifier.urihttp://hdl.handle.net/10072/60314
dc.description.abstractBiomarkers of the age of mosquitoes are required to determine the risk of transmission of various pathogens as each pathogen undergoes a period of extrinsic incubation in the mosquito host. Using the 2-D Difference Gel Electrophoresis (2-D DIGE) procedure, we investigated the abundance of up to 898 proteins from the Yellow Fever and dengue virus vector, Aedes aegypti, during ageing. By applying a mixed-effects model of protein expression, we identified five common patterns of abundance change during ageing and demonstrated an age-related decrease in variance for four of these. This supported a search for specific proteins with abundance changes that remain tightly associated with ageing for use as ageing biomarkers. Using MALDI-TOF/TOF mass spectrometry we identified ten candidate proteins that satisfied strict biomarker discovery criteria (identified in two out of three multivariate analysis procedures and in two cohorts of mosquitoes). We validated the abundances of the four most suitable candidates (Actin depolymerising factor; ADF, Eukaryotic initiation factor 5A; eIF5A, insect cuticle protein Q17LN8, and Anterior fat body protein; AFP) using semi-quantitative Western analysis of individual mosquitoes of six ages. The redox-response protein Manganese superoxide dismutase (SOD2) and electron shuttling protein Electron transfer oxidoreductase (ETO) were subject to post-translational modifications affecting their charge states with potential effects on function. For the four candidates we show remarkably consistent decreases in abundance during ageing, validating initial selections. In particular, the abundance of AFP is an ideal biomarker candidate for whether a female mosquito has lived long enough to be capable of dengue virus transmission. We have demonstrated proteins to be a suitable class of ageing biomarkers in mosquitoes and have identified candidates for epidemiological studies of dengue and the evaluation of new disease reduction projects targeting mosquito longevity.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_US
dc.format.extent4942583 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglishen_US
dc.publisherPublic Library of Scienceen_US
dc.publisher.placeUnited Statesen_US
dc.relation.ispartofstudentpublicationNen_US
dc.relation.ispartofpagefrome58656-1en_US
dc.relation.ispartofpagetoe58656-10en_US
dc.relation.ispartofissue3en_US
dc.relation.ispartofjournalPloS Oneen_US
dc.relation.ispartofvolume8en_US
dc.rights.retentionYen_US
dc.subject.fieldofresearchMedical Microbiology not elsewhere classifieden_US
dc.subject.fieldofresearchcode110899en_US
dc.titleProteomic Biomarkers for Ageing the Mosquito Aedes aegypti to Determine Risk of Pathogen Transmissionen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
dcterms.licensehttp://www.plos.org/journals/license.htmlen_US
gro.rights.copyright© 2013 Hugo et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License CCAL. (http://www.plos.org/journals/license.html)en_US
gro.hasfulltextFull Text
gro.griffith.authorSikulu, Maggy TL.


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