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dc.contributor.authorE. Hugo, Leon
dc.contributor.authorMonkman, James
dc.contributor.authorA. Dave, Keyur
dc.contributor.authorF. Wockner, Leesa
dc.contributor.authorW. Birrell, Geoff
dc.contributor.authorL. Norris, Emma
dc.contributor.authorJ. Kienzle, Vivian
dc.contributor.authorSikulu, Maggy
dc.contributor.authorRyan, Peter A.
dc.contributor.authorJ. Gorman, Jeffery
dc.contributor.authorH. Kay, Brian
dc.date.accessioned2017-05-03T13:13:37Z
dc.date.available2017-05-03T13:13:37Z
dc.date.issued2013
dc.date.modified2014-06-16T04:42:27Z
dc.identifier.issn19326203
dc.identifier.doi10.1371/journal.pone.0058656
dc.identifier.urihttp://hdl.handle.net/10072/60314
dc.description.abstractBiomarkers of the age of mosquitoes are required to determine the risk of transmission of various pathogens as each pathogen undergoes a period of extrinsic incubation in the mosquito host. Using the 2-D Difference Gel Electrophoresis (2-D DIGE) procedure, we investigated the abundance of up to 898 proteins from the Yellow Fever and dengue virus vector, Aedes aegypti, during ageing. By applying a mixed-effects model of protein expression, we identified five common patterns of abundance change during ageing and demonstrated an age-related decrease in variance for four of these. This supported a search for specific proteins with abundance changes that remain tightly associated with ageing for use as ageing biomarkers. Using MALDI-TOF/TOF mass spectrometry we identified ten candidate proteins that satisfied strict biomarker discovery criteria (identified in two out of three multivariate analysis procedures and in two cohorts of mosquitoes). We validated the abundances of the four most suitable candidates (Actin depolymerising factor; ADF, Eukaryotic initiation factor 5A; eIF5A, insect cuticle protein Q17LN8, and Anterior fat body protein; AFP) using semi-quantitative Western analysis of individual mosquitoes of six ages. The redox-response protein Manganese superoxide dismutase (SOD2) and electron shuttling protein Electron transfer oxidoreductase (ETO) were subject to post-translational modifications affecting their charge states with potential effects on function. For the four candidates we show remarkably consistent decreases in abundance during ageing, validating initial selections. In particular, the abundance of AFP is an ideal biomarker candidate for whether a female mosquito has lived long enough to be capable of dengue virus transmission. We have demonstrated proteins to be a suitable class of ageing biomarkers in mosquitoes and have identified candidates for epidemiological studies of dengue and the evaluation of new disease reduction projects targeting mosquito longevity.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent4942583 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherPublic Library of Science
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrome58656-1
dc.relation.ispartofpagetoe58656-10
dc.relation.ispartofissue3
dc.relation.ispartofjournalPloS One
dc.relation.ispartofvolume8
dc.rights.retentionY
dc.subject.fieldofresearchMedical Microbiology not elsewhere classified
dc.subject.fieldofresearchcode110899
dc.titleProteomic Biomarkers for Ageing the Mosquito Aedes aegypti to Determine Risk of Pathogen Transmission
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://www.plos.org/journals/license.html
gro.rights.copyright© 2013 Hugo et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License CCAL. (http://www.plos.org/journals/license.html)
gro.hasfulltextFull Text
gro.griffith.authorSikulu, Maggy TL.


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