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  • Gene expression in human alcoholism: Microarray analysis of frontal cortex

    Author(s)
    Lewohl, JM
    Wang, L
    Miles, MF
    Zhang, L
    Dodd, PR
    Harris, RA
    Griffith University Author(s)
    Lewohl, Joanne M.
    Dodd, Peter
    Year published
    2000
    Metadata
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    Abstract
    Background: Changes in brain gene expression are thought to be responsible for the tolerance, dependence, and neurotoxicity produced by chronic alcohol abuse, but there has been no large scale study of gene expression in human alcoholism. Methods: RNA was extracted from postmortem samples of superior frontal cortex of alcoholics and nonalcoholics. Relative levels of RNA were determined by array techniques. We used both cDNA and oligonucleotide microarrays to provide coverage of a large number of genes and to allow cross-validation for those genes represented on both types of arrays. Results: Expression levels were determined ...
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    Background: Changes in brain gene expression are thought to be responsible for the tolerance, dependence, and neurotoxicity produced by chronic alcohol abuse, but there has been no large scale study of gene expression in human alcoholism. Methods: RNA was extracted from postmortem samples of superior frontal cortex of alcoholics and nonalcoholics. Relative levels of RNA were determined by array techniques. We used both cDNA and oligonucleotide microarrays to provide coverage of a large number of genes and to allow cross-validation for those genes represented on both types of arrays. Results: Expression levels were determined for over 4000 genes and 163 of these were found to differ by 40% or more between alcoholics and nonalcoholics. Analysis of these changes revealed a selective reprogramming of gene expression in this brain region, particularly for myelin-related genes which were down-regulated in the alcoholic samples. In addition, cell cycle genes and several neuronal genes were changed in expression. Conclusions: These gene expression changes suggest a mechanism for the loss of cerebral white matter in alcoholics as well as alterations that may lead to the neurotoxic actions of ethanol.
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    Journal Title
    Alcoholism, clinical and experimental research
    Volume
    24
    Issue
    12
    DOI
    https://doi.org/10.1111/j.1530-0277.2000.tb01993.x
    Subject
    Clinical Sciences
    Neurosciences
    Psychology
    Publication URI
    http://hdl.handle.net/10072/60379
    Collection
    • Journal articles

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