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  • IgG N-glycans as potential biomarkers for determining galactose tolerance in Classical Galactosaemia

    Author(s)
    Coss, KP
    Byrne, JC
    Coman, DJ
    Adamczyk, B
    Abrahams, JL
    Saldova, R
    Brown, AY
    Walsh, O
    Hendroff, U
    Carolan, C
    Rudd, PM
    Treacy, EP
    Griffith University Author(s)
    Coman, Dave J.
    Abrahams, Jodie L.
    Year published
    2012
    Metadata
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    Abstract
    N-glycan processing and assembly defects have been demonstrated in untreated and partially treated patients with Classical Galactosaemia. These defects may contribute to the ongoing pathophysiology of this disease. The aim of this study was to develop an informative method of studying differential galactose tolerance levels and diet control in individuals with Galactosaemia, compared to the standard biochemical markers. Ten Galactosaemia adults with normal intellectual outcomes were analyzed in the study. Five subjects followed galactose liberalization, increments of 300 mg to 4000 mg/day over 16 weeks, and were compared ...
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    N-glycan processing and assembly defects have been demonstrated in untreated and partially treated patients with Classical Galactosaemia. These defects may contribute to the ongoing pathophysiology of this disease. The aim of this study was to develop an informative method of studying differential galactose tolerance levels and diet control in individuals with Galactosaemia, compared to the standard biochemical markers. Ten Galactosaemia adults with normal intellectual outcomes were analyzed in the study. Five subjects followed galactose liberalization, increments of 300 mg to 4000 mg/day over 16 weeks, and were compared to five adult Galactosaemia controls on a galactose restricted diet. All study subjects underwent clinical and biochemical monitoring of red blood cell galactose-1-phosphate (RBC Gal-1-P) and urinary galactitol levels. Serum N-glycans were isolated and analyzed by normal phase high-performance liquid chromatography (NP-HPLC) with galactosylation of IgG used as a specific biomarker of galactose tolerance. IgG N-glycan profiles showed consistent individual alterations in response to diet liberalization. The individual profiles were improved for all, but one study subject, at a galactose intake of 1000 mg/day, with decreases in agalactosylated (G0) and increases in digalactosylated (G2) N-glycans. We conclude that IgG N-glycan profiling is an improved method of monitoring variable galactosylation and determining individual galactose tolerance in Galactosaemia compared to the standard methods.
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    Journal Title
    Molecular Genetics and Metabolism
    Volume
    105
    Issue
    2
    DOI
    https://doi.org/10.1016/j.ymgme.2011.10.018
    Subject
    Clinical sciences
    Publication URI
    http://hdl.handle.net/10072/60492
    Collection
    • Journal articles

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