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dc.contributor.authorTiralongo, J
dc.contributor.authorSchmid, H
dc.contributor.authorThun, R
dc.contributor.authorIwersen, M
dc.contributor.authorSchauer, R
dc.date.accessioned2006-07-28
dc.date.accessioned2014-06-19T22:44:17Z
dc.date.accessioned2017-03-02T00:11:16Z
dc.date.available2017-03-02T00:11:16Z
dc.date.issued2000
dc.date.modified2014-06-19T22:44:17Z
dc.identifier.issn0282-0080
dc.identifier.doi10.1023/A:1010965128335
dc.identifier.urihttp://hdl.handle.net/10072/60695
dc.description.abstractMicrosomes prepared from equine submandibular glands and incubated with tritium-labelled AcCoA incorporated acid-insoluble radioactivity in a manner dependent on time, protein, membrane integrity and AcCoA concentration, with incorporation being optimal at 37°C and pH 6.6. Under the experimental conditions used a K M of 32.1 μM for AcCoA and a Vmax of 1.2 pmol/mg protein x min was obtained. The incorporation of acid-insoluble radioactivity was also inhibited by CoA in a competitive manner (K i=240 μM), as well as by para-chloromercuribenzoate, 3′-dephospho-CoA, 5′-IDP, 5′-ADP, ß-NAD and 4,4′-diisothiocyanatostilbene-2,2′-disulfonate. We demonstrate here that this incorporation of radioactivity into endogenous sialic acid is due to the action of an AcCoA:sialate-4-O-acetyltransferase [EC 2.3.1.44]. Radio thin-layer chromatography analyses of propionic acid-released sialic acids showed that the incorporation of radioactivity correlated with the formation of a radiolabelled species that co-migrated with authentic Neu4,5Ac2. Saponification experiments using NaOH, mouse hepatitis virus strain S and Influenza C/JJ/50 virus also showed that the transfer of [3H]acetyl groups from [3H]AcCoA to endogenous sialic acid acceptors was occurring exclusively at carbon 4 of the pyranose ring.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSpringer
dc.publisher.placeUS
dc.relation.ispartofpagefrom849
dc.relation.ispartofpageto858
dc.relation.ispartofjournalGlycoconjugate Journal
dc.relation.ispartofvolume17
dc.subject.fieldofresearchBiochemistry and Cell Biology
dc.subject.fieldofresearchMedical Microbiology
dc.subject.fieldofresearchNeurosciences
dc.subject.fieldofresearchcode0601
dc.subject.fieldofresearchcode1108
dc.subject.fieldofresearchcode1109
dc.titleCharacterisation of the enzymatic 4-O-acetylation of sialic acids in microsomes from equine submandibular glands
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codec1x
gro.facultyInstitute for Glycomics
gro.hasfulltextNo Full Text
gro.griffith.authorTiralongo, Joe


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