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  • Quantitative PCR confirms culture as the gold standard for detection of lower airway infection by nontypeable Haemophilus influenzae in Australian Indigenous children with bronchiectasis

    Author(s)
    Hare, Kim M
    Marsh, Robyn L
    Binks, Michael J
    Grimwood, Keith
    Pizzutto, Susan J
    Leach, Amanda J
    Chang, Anne B
    Smith-Vaughan, Heidi C
    Griffith University Author(s)
    Smith-Vaughan, Heidi
    Grimwood, Keith
    Year published
    2013
    Metadata
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    Abstract
    Correlation was observed between quantitative PCR and semi-quantitative culture for definition of Haemophilus influenzae infection in bronchoalveolar lavage specimens from 81 children with bronchiectasis. However, qPCR data correlated less well with airway neutrophilia, and supports continued use of culture as the gold standard for defining H. influenzae lower airway infection.Correlation was observed between quantitative PCR and semi-quantitative culture for definition of Haemophilus influenzae infection in bronchoalveolar lavage specimens from 81 children with bronchiectasis. However, qPCR data correlated less well with airway neutrophilia, and supports continued use of culture as the gold standard for defining H. influenzae lower airway infection.
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    Journal Title
    Journal of Microbiological Methods
    Volume
    92
    DOI
    https://doi.org/10.1016/j.mimet.2012.12.013
    Subject
    Cardiorespiratory Medicine and Haematology not elsewhere classified
    Microbiology
    Medical Microbiology
    Publication URI
    http://hdl.handle.net/10072/60789
    Collection
    • Journal articles

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    • Gold Coast
    • Logan
    • Brisbane - Queensland, Australia
    First Peoples of Australia
    • Aboriginal
    • Torres Strait Islander