Show simple item record

dc.contributor.authorAl-Jiffry, BO
dc.contributor.authorMeedeniya, AC
dc.contributor.authorChen, JW
dc.contributor.authorToouli, J
dc.contributor.authorSaccone, GT
dc.contributor.editorSchmidt, W.E.
dc.date.accessioned2006-08-06
dc.date.accessioned2014-06-27T01:17:10Z
dc.date.accessioned2017-03-02T00:40:12Z
dc.date.available2017-03-02T00:40:12Z
dc.date.issued2001
dc.date.modified2014-06-27T01:17:10Z
dc.identifier.issn0167-0115
dc.identifier.doi10.1016/S0167-0115(01)00302-0
dc.identifier.urihttp://hdl.handle.net/10072/60954
dc.description.abstractBACKGROUND: Endothelin-1 (ET-1) stimulates guinea pig gallbladder (GB) muscle strip contractility; however, the role and source of ET-1 in the GB remains to be elucidated. AIMS: To determine the effect of ET-1 on human and possum GB muscle strip contractility and evaluate whether ET-1 is present in GB tissue. METHODS: GB muscle strips were mounted in organ baths to measure isometric tension. ET-1 was added cumulatively with and without pretreatment with the neural blocker tetrodotoxin (TTX) or the ET receptor antagonists BQ-123, BQ-788, and tezosentan. Immunohistochemical localization of ET was performed on freshly fixed and cultured GBs. RESULTS: ET-1 induced concentration-dependent increases in tone in human and possum GB strips (p<0.05). This response was unaffected by BQ-123, BQ-788, and TTX but antagonized by BQ-123+BQ-788 in the human tissue only. Tezosentan (10(-4) mol/l) blocked the ET-1-induced response in human and possum GB strips (p<0.001). Although ET immunoreactivity was absent in freshly fixed possum GB, immunoreactivity was observed in the GB epithelium of freshly fixed human tissue and in both possum and human tissue following 24 h of organ culture. CONCLUSION: ET-1 acts directly on human and possum GB smooth muscle producing contractions, possibly via ET-B receptors. ET may be present under pathophysiological conditions altering GB function.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageeng
dc.publisherThomson Scientific
dc.publisher.placeBochum, Germany
dc.relation.ispartofpagefrom31
dc.relation.ispartofpageto39
dc.relation.ispartofissue1
dc.relation.ispartofjournalRegulatory Peptides
dc.relation.ispartofvolume102
dc.subject.fieldofresearchMedical and Health Sciences
dc.subject.fieldofresearchcode11
dc.titleEndothelin-1 induces contraction of human and Australian possum gallbladder in vitro
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codec1x
gro.facultyOther
gro.hasfulltextNo Full Text
gro.griffith.authorMeedeniya, Adrian C.


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record