Micropropagation of moringa oleifera

Abstract

Moringa oleifera (moringa) is a fast growing tree and is recognized for its high nutritional value. Because it is a rich source of essential human vitamins and minerals, it has the potential to help alleviate malnutrition worldwide. Experiments were conducted to develop a protocol for micropropagation of moringa. Shoot growth was optimal on shoot growth medium containing high concentrations of Drew and Smith (1986) macro- and micro-nutrients (DSH), MS vitamins and 1 占kinetin. Root initiation and growth was optimal on medium containing medium concentrations of Drew and Smith (1986) macro- and micro-nutrients (DSM) plus 10 占indole-3-acetic acid (IAA) for two days before transfer to DSM, MS vitamins and 1 占kinetin. Experiments on the addition of various concentrations of kinetin, riboflavin and potassium were conducted in an attempt to optimize root growth and minimize callus production on shoots in vitro. Transfer of both apical and axillary bud explants to medium containing 1 占kinetin and 10 占riboflavin after two days on medium containing 10 占IAA, minimized callus production. Increased potassium concentration decreased shoot growth and number of roots per shoot. Increased potassium concentration in the media decreased callus production and decreased root growth in the presence of DSH; and, increased root growth in medium containing DSM. Further experiments will be conducted to optimize growth of roots and minimize callus growth to facilitate acclimatization.