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dc.contributor.authorGarvican, Laura A.
dc.contributor.authorBurge, Caroline M.
dc.contributor.authorCox, Amanda J.
dc.contributor.authorClark, Sally A.
dc.contributor.authorMartin, David T.
dc.contributor.authorGore, Christopher J.
dc.date.accessioned2017-05-03T11:59:33Z
dc.date.available2017-05-03T11:59:33Z
dc.date.issued2010
dc.date.modified2014-07-21T05:10:30Z
dc.identifier.issn09580670
dc.identifier.doi10.1113/expphysiol.2010.054031
dc.identifier.urihttp://hdl.handle.net/10072/61430
dc.description.abstractThe uptake and distribution of CO throughout the circulatory system during two different methods of CO rebreathing (2 min ‘Schmidt’ and 40 min ‘Burge’ methods) were determined in nine healthy volunteers. Specifically, the impact of (i) differences in circulatory mixing time (tmix), (ii) CO diffusion to myoglobin (Mb) and (iii) CO wash-out was assessed on calculated haemoglobin mass (Hbmass). Arterial (a), muscle venous (vm) and capillary samples (c) were obtained simultaneously at 0, 1, 2, 3.5, 5, 7.5, 10, 12.5, 15, 20, 30 and 40 min for determination of carboxyhaemoglobin (HbCO). Carbon monoxide wash-out was measured from expired air following rebreathing. The rate of CO diffusion to Mb was calculated using the change in HbCO after tmix, and the rate of CO wash-out. In both methods, HbCOa and HbCOc followed a near-identical time course, peaking within the first 2 min and decreasing thereafter. The HbCOvm increased slowly and was significantly lower at 1, 2 and 3.5 min in both methods (P < 0.01). The HbCOa peaked significantly higher in the Schmidt method (P= 0.03). Circulatory mixing had occurred by 10 min in most but not all subjects. The rate of CO wash-out was 0.25 ± 0.13 ml min−1 in the Schmidt and 0.25 ± 0.16 ml min−1 in the Burge method. The rate of CO diffusion to Mb was 0.22 ± 0.11 and 0.16 ± 0.13 ml min−1 (P= 0.63) in Schmidt and Burge methods, respectively. Inhalation of a CO bolus during the Schmidt method results in faster HbCOa uptake but does not greatly shorten tmix or influence rates of CO wash-out and flux to Mb. The calculated Hbmass depends substantially on the plateau level of HbCO; therefore, it is paramount to ensure HbCO is mixed completely prior to blood sampling, as well as accounting for potential within-subject alterations of CO exhalation and CO flux to Mb.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherWiley-Blackwell Publishing
dc.publisher.placeUnited Kingdom
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom1156
dc.relation.ispartofpageto1166
dc.relation.ispartofissue12
dc.relation.ispartofjournalExperimental Physiology
dc.relation.ispartofvolume95
dc.rights.retentionY
dc.subject.fieldofresearchMedical Physiology not elsewhere classified
dc.subject.fieldofresearchPhysiology
dc.subject.fieldofresearchHuman Movement and Sports Sciences
dc.subject.fieldofresearchMedical Physiology
dc.subject.fieldofresearchcode111699
dc.subject.fieldofresearchcode0606
dc.subject.fieldofresearchcode1106
dc.subject.fieldofresearchcode1116
dc.titleCarbon monoxide uptake kinetics of arterial, venous and capillary blood during CO rebreathing
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorCox, Amanda J.


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