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  • The generation and properties of human macrophage populations from hemopoietic stem cells

    Author(s)
    Way, Kerrie J
    Dinh, Hang
    Keene, Martin R
    White, Kirby E
    Clanchy, Felix IL
    Lusby, Patricia
    Roiniotis, John
    Cook, Andrew D
    Cassady, A Ian
    Curtis, David J
    Hamilton, John A
    Griffith University Author(s)
    Cassady, Ian
    Year published
    2009
    Metadata
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    Abstract
    Information about the development and function of human macrophage lineage populations, such as osteoclasts, is limited because of the lack of defined in vitro systems for their large-scale generation. Two M-CSF-containing cytokine cocktails were found under serum-free conditions to expand dramatically and to differentiate over time human CD34+ hemopoietic stem cells into nonadherent and adherent macrophage populations. These populations exhibited increasing degrees of maturity over a 3-week period characterized by morphology, surface marker expression (CD11b, CD86, CD64, CD14, and c-Fms), phagocytic function, and gene-expression ...
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    Information about the development and function of human macrophage lineage populations, such as osteoclasts, is limited because of the lack of defined in vitro systems for their large-scale generation. Two M-CSF-containing cytokine cocktails were found under serum-free conditions to expand dramatically and to differentiate over time human CD34+ hemopoietic stem cells into nonadherent and adherent macrophage populations. These populations exhibited increasing degrees of maturity over a 3-week period characterized by morphology, surface marker expression (CD11b, CD86, CD64, CD14, and c-Fms), phagocytic function, and gene-expression profiling using quantitative PCR and microarray analysis (principal component analysis, k-means clustering, and gene ontology classification). As assessed by the last criterion, the adherent population obtained at 3 weeks from the one protocol tested had high similarity to the well-studied peripheral blood monocyte-derived macrophages. The one population tested could be induced to differentiate into osteoclasts in the presence of M-CSF and receptor activator of NF-?B ligand, as judged by morphology, gene expression, and bone-resorbing ability. In addition to the large numbers of macrophage lineage cells able to be produced, this replicating system may be suitable for the molecular analysis of macrophage lineage commitment and progression and for gene targeting and delivery.
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    Journal Title
    Journal of Leukocyte Biology
    Volume
    85
    Issue
    5
    DOI
    https://doi.org/10.1189/jlb.1108689
    Subject
    Medical and Health Sciences not elsewhere classified
    Immunology not elsewhere classified
    Biochemistry and Cell Biology
    Immunology
    Publication URI
    http://hdl.handle.net/10072/61448
    Collection
    • Journal articles

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