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  • An Assessment of MMP and TIMP Gene Expression in Cell Lines and Stroma - Tumour Differences in Microdissected Breast Cancer Biopsies

    Author(s)
    Mellick, AS
    Blackmore, D
    Weinstein, SR
    Griffiths, LR
    Griffith University Author(s)
    Griffiths, Lyn
    Weinstein, Stephen R.
    Blackmore, Daneia
    Mellick, Albert S.
    Year published
    2003
    Metadata
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    Abstract
    To examine matrix metalloproteinase (MMP) and tissue inhibitor of metalloproteinases (TIMP) mRNA levels in archival breast cancer biopsies, we employed microdissection to separate tumour tissue from the surrounding breast tissue, or stroma and RT-PCR to determine gross qualitative and small quantitative differences in the patterns of expression. In this study, a significant correlation (p < 0.05, by Mann-Whitney U analysis) between TIMP-2 expression and lymph node involvement was identified, while MMP-11 and TIMP-1 expression patterning also significantly (p < 0.05) differed between those tumours showing calcification and ...
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    To examine matrix metalloproteinase (MMP) and tissue inhibitor of metalloproteinases (TIMP) mRNA levels in archival breast cancer biopsies, we employed microdissection to separate tumour tissue from the surrounding breast tissue, or stroma and RT-PCR to determine gross qualitative and small quantitative differences in the patterns of expression. In this study, a significant correlation (p < 0.05, by Mann-Whitney U analysis) between TIMP-2 expression and lymph node involvement was identified, while MMP-11 and TIMP-1 expression patterning also significantly (p < 0.05) differed between those tumours showing calcification and those that did not. When compared by Spearmans' correlation analysis, a significant association (p < 0.05, = 0.404) was identified in the pattern of MMP-2 and MMP-9 gene expression. In this study, the use of microdissection and a systematic strategy of RT-PCR analysis have allowed us to investigate localized MMP and MMP inhibitor expression within breast tumours. We have identified patterns of gene expression that may further reveal aspects of breast carcinogenesis, and a robust method for examining changes in clinically important genes using archival biopsies and across stroma-tumour boundaries.
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    Journal Title
    Tumor Biology
    Volume
    24
    Issue
    5
    Publisher URI
    https://www.karger.com/Article/Abstract/76140
    DOI
    https://doi.org/10.1159/000076140
    Subject
    Clinical sciences
    Publication URI
    http://hdl.handle.net/10072/6178
    Collection
    • Journal articles

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