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dc.contributor.authorHatzirodos, Nicholas
dc.contributor.authorHummitzsch, Katja
dc.contributor.authorIrving-Rodgers, Helen F
dc.contributor.authorHarland, Margaret L
dc.contributor.authorMorris, Stephanie E
dc.contributor.authorRodgers, Raymond J
dc.date.accessioned2018-03-26T01:31:15Z
dc.date.available2018-03-26T01:31:15Z
dc.date.issued2014
dc.date.modified2014-08-05T23:02:32Z
dc.identifier.issn1471-2164
dc.identifier.doi10.1186/1471-2164-15-40
dc.identifier.urihttp://hdl.handle.net/10072/61880
dc.description.abstractBackground The major function of the ovary is to produce oocytes for fertilisation. Oocytes mature in follicles surrounded by nurturing granulosa cells and all are enclosed by a basal lamina. During growth, granulosa cells replicate and a large fluid-filled cavity (the antrum) develops in the centre. Only follicles that have enlarged to over 10 mm can ovulate in cows. In mammals, the number of primordial follicles far exceeds the numbers that ever ovulate and atresia or regression of follicles is a mechanism to regulate the number of oocytes ovulated and to contribute to the timing of ovulation. To better understand the molecular basis of follicular atresia, we undertook transcriptome profiling of granulosa cells from healthy (n = 10) and atretic (n = 5) bovine follicles at early antral stages (< 5 mm). Results Principal Component Analysis (PCA) and hierarchical classification of the signal intensity plots for the arrays showed primary clustering into two groups, healthy and atretic. These analyses and size-frequency plots of coefficients of variation of signal intensities revealed that the healthy follicles were more heterogeneous. Examining the differentially-expressed genes the most significantly affected functions in atretic follicles were cell death, organ development, tissue development and embryonic development. The overall processes influenced by transcription factor gene TP53 were predicted to be activated, whereas those of MYC were inhibited on the basis of known interactions with the genes in our dataset. The top ranked canonical pathway contained signalling molecules common to various inflammatory/fibrotic pathways such as the transforming growth factor-ߠand tumour necrosis factor-a pathways. The two most significant networks also reflect this pattern of tissue remodelling/fibrosis gene expression. These networks also contain molecules which are present in the canonical pathways of hepatic fibrosis/hepatic stellate cell activation and transforming growth factor-ߠsignalling and were up regulated. Conclusions Small healthy antral follicles, which have a number of growth outcomes, exhibit greater variability in gene expression, particularly in genes associated with cell division and other growth-related functions. Atresia, on the other hand, not only involves cell death but clearly is an active process similar to wound healing.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent1721437 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherBioMed Central
dc.publisher.placeUnited Kingdom
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom40-1
dc.relation.ispartofpageto40-26
dc.relation.ispartofjournalBMC Genomics
dc.relation.ispartofvolume15
dc.rights.retentionY
dc.subject.fieldofresearchBiological sciences
dc.subject.fieldofresearchCell development, proliferation and death
dc.subject.fieldofresearchInformation and computing sciences
dc.subject.fieldofresearchBiomedical and clinical sciences
dc.subject.fieldofresearchcode31
dc.subject.fieldofresearchcode310102
dc.subject.fieldofresearchcode46
dc.subject.fieldofresearchcode32
dc.titleTranscriptome profiling of granulosa cells from bovine ovarian follicles during atresia
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by/2.0
gro.description.notepublicPage numbers are not for citation purposes. Instead, this article has the unique article number of 40.
gro.rights.copyright© 2014 Hatzirodos et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorIrving-Rodgers, Helen F.


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