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dc.contributor.authorRose'Meyer, RB
dc.contributor.authorMellick, AS
dc.contributor.authorGarnham, BG
dc.contributor.authorHarrison, GJ
dc.contributor.authorMassa, HM
dc.contributor.authorGriffiths, LR
dc.contributor.editorF G Wouterlood
dc.date.accessioned2017-05-03T11:37:01Z
dc.date.available2017-05-03T11:37:01Z
dc.date.issued2003
dc.date.modified2008-11-30T22:45:12Z
dc.identifier.issn1385-299X
dc.identifier.doi10.1016/S1385-299X(02)00219-2
dc.identifier.urihttp://hdl.handle.net/10072/6190
dc.description.abstractIn our laboratory we have developed a quantitative-polymerase chain reaction (Q-PCR) strategy to examine the differential expression of adenosine receptor (ADOR), A1, A2A, A2B and A3, and estrogen receptors (ER) a and ߮ Brain and uterine mRNA were first used to optimise specific amplification conditions prior to SYBR Green I real time analysis of receptor subtype expression. SYBR Green I provided a convenient and sensitive means of examining specific PCR amplification product in real time, and allowed the generation of standard curves from which relative receptor abundance could be determined. Real time Q-PCR analysis was then performed, to examine changes in receptor expression levels in brains of adult female Wistar rats 3-month post ovariectomy. Comparison with sham-operated age-matched control rats demonstrated both comparative and absolute-copy number changes in receptor levels. Evaluation of both analytical methods investigated 18S rRNA as an internal reference for comparative gene expression analysis in the brain. The results of this study revealed preferential repression of ADORA2A (>4-fold down) and consistent (>2-fold) down-regulation of ADORA1, ADORA3, and ER-߬ following ovariectomy. No change was found in ADORA2B or ER-a. Analysis of absolute copy number in this study revealed a correlation between receptor expression in response to ovariectomy, and relative receptor subtype abundance in the brain.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.publisher.placeNetherlands
dc.publisher.urihttp://www.elsevier.com/wps/find/journaldescription.cws_home/622287/description#description
dc.relation.ispartofpagefrom9
dc.relation.ispartofpageto18
dc.relation.ispartofjournalBrain Research Protocols
dc.relation.ispartofvolume11
dc.subject.fieldofresearchNeurosciences
dc.subject.fieldofresearchCognitive and computational psychology
dc.subject.fieldofresearchBiological psychology
dc.subject.fieldofresearchcode3209
dc.subject.fieldofresearchcode5204
dc.subject.fieldofresearchcode5202
dc.titleThe measurement of adenosine and estrogen receptor expression in rat brains following ovariectomy using quantitative PCR analysis
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Health, School of Medical Science
gro.rights.copyright© 2003 Elsevier : Reproduced in accordance with the copyright policy of the publisher : This journal is available online - use hypertext links
gro.date.issued2003
gro.hasfulltextNo Full Text
gro.griffith.authorHarrison, Glenn J.
gro.griffith.authorMassa, Helen M.
gro.griffith.authorRose'Meyer, Roselyn B.


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