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  • Arthritogenic alphaviral infection perturbs osteoblast function and triggers pathologic bone loss

    Author(s)
    Chen, Weiqiang
    Foo, Suan-Sin
    Rulli, Nestor E
    Taylor, Adam
    Sheng, Kuo-Ching
    Herrero, Lara J
    Herring, Belinda L
    Lidbury, Brett A
    Li, Rachel W
    Walsh, Nicole C
    Sims, Natalie A
    Smith, Paul N
    Mahalingam, Suresh
    Griffith University Author(s)
    Mahalingam, Suresh
    Herrero, Lara J.
    Taylor, Adam
    Year published
    2014
    Metadata
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    Abstract
    Arthritogenic alphaviruses including Ross River virus (RRV), Sindbis virus, and chikungunya virus cause worldwide outbreaks of musculoskeletal disease. The ability of alphaviruses to induce bone pathologies remains poorly defined. Here we show that primary human osteoblasts (hOBs) can be productively infected by RRV. RRV-infected hOBs produced high levels of inflammatory cytokine including IL-6. The RANKL/OPG ratio was disrupted in the synovial fluid of RRV patients, and this was accompanied by an increase in serum Tartrate-resistant acid phosphatase 5b (TRAP5b) levels. Infection of bone cells with RRV was validated using ...
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    Arthritogenic alphaviruses including Ross River virus (RRV), Sindbis virus, and chikungunya virus cause worldwide outbreaks of musculoskeletal disease. The ability of alphaviruses to induce bone pathologies remains poorly defined. Here we show that primary human osteoblasts (hOBs) can be productively infected by RRV. RRV-infected hOBs produced high levels of inflammatory cytokine including IL-6. The RANKL/OPG ratio was disrupted in the synovial fluid of RRV patients, and this was accompanied by an increase in serum Tartrate-resistant acid phosphatase 5b (TRAP5b) levels. Infection of bone cells with RRV was validated using an established RRV murine model. In wild-type mice, infectious virus was detected in the femur, tibia, patella, and foot, together with reduced bone volume in the tibial epiphysis and vertebrae detected by microcomputed tomographic (僔) analysis. The RANKL/OPG ratio was also disrupted in mice infected with RRV; both this effect and the bone loss were blocked by treatment with an IL-6 neutralizing antibody. Collectively, these findings provide previously unidentified evidence that alphavirus infection induces bone loss and that OBs are capable of producing proinflammatory mediators during alphavirus-induced arthralgia. The perturbed RANKL/OPG ratio in RRV-infected OBs may therefore contribute to bone loss in alphavirus infection.
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    Journal Title
    Proceedings of the National Academy of Sciences of the United States of America
    Volume
    111
    Issue
    16
    DOI
    https://doi.org/10.1073/pnas.1318859111
    Subject
    Medical virology
    Publication URI
    http://hdl.handle.net/10072/61990
    Collection
    • Journal articles

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