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dc.contributor.authorIrving-Rodgers, HF
dc.contributor.authorChoong, FJ
dc.contributor.authorHummitzsch, K
dc.contributor.authorParish, CR
dc.contributor.authorRodgers, RJ
dc.contributor.authorSimeonovic, CJ
dc.date.accessioned2017-05-03T16:13:09Z
dc.date.available2017-05-03T16:13:09Z
dc.date.issued2014
dc.date.modified2014-08-08T04:04:50Z
dc.identifier.issn0963-6897
dc.identifier.doi10.3727/096368912X659880
dc.identifier.urihttp://hdl.handle.net/10072/62052
dc.description.abstractThe isolation of islets by collagenase digestion can cause damage and impact the efficiency of islet engraftment and function. In this study, we assessed the basement membranes (BMs) of mouse pancreatic islets as a molecular biomarker for islet integrity, damage after isolation, and islet repair in vitro as well as in the absence or presence of an immune response after transplantation. Immunofluorescence staining of BM matrix proteins and the endothelial cell marker platelet endothelial cell adhesion molecule-1 (PECAM-1) was performed on pancreatic islets in situ, isolated islets, islets cultured for 4 days, and islet grafts at 3-10 days posttransplantation. Flow cytometry was used to investigate the expression of BM matrix proteins in isolated islet ߭cells. The islet BM, consisting of collagen type IV and components of Engelbreth-Holm-Swarm (EHS) tumor laminin 111, laminin a2, nidogen-2, and perlecan in pancreatic islets in situ, was completely lost during islet isolation. It was not reestablished during culture for 4 days. Peri- and intraislet BM restoration was identified after islet isotransplantation and coincided with the migration pattern of PECAM-1+ vascular endothelial cells (VECs). After islet allotransplantation, the restoration of VEC-derived peri-islet BMs was initiated but did not lead to the formation of the intraislet vasculature. Instead, an abnormally enlarged peri-islet vasculature developed, coinciding with islet allograft rejection. The islet BM is a sensitive biomarker of islet damage resulting from enzymatic isolation and of islet repair after transplantation. After transplantation, remodeling of both peri- and intraislet BMs restores ߭cell-matrix attachment, a recognized requirement for ߭cell survival, for isografts but not for allografts. Preventing isolation-induced islet BM damage would be expected to preserve the intrinsic barrier function of islet BMs, thereby influencing both the effector mechanisms required for allograft rejection and the antirejection strategies needed for allograft survival.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent1649006 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherCognizant Communication Corporation
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom59
dc.relation.ispartofpageto72
dc.relation.ispartofissue1
dc.relation.ispartofjournalCell Transplantation
dc.relation.ispartofvolume23
dc.rights.retentionY
dc.subject.fieldofresearchAnimal Structure and Function
dc.subject.fieldofresearchAnimal Physiology - Cell
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchTechnology
dc.subject.fieldofresearchMedical and Health Sciences
dc.subject.fieldofresearchcode060807
dc.subject.fieldofresearchcode060602
dc.subject.fieldofresearchcode06
dc.subject.fieldofresearchcode10
dc.subject.fieldofresearchcode11
dc.titlePancreatic islet basement membrane loss and remodeling after mouse islet isolation and transplantation: impact for allograft rejection
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyright© 2012 Cognizant Communication Corporation. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
gro.hasfulltextFull Text
gro.griffith.authorIrving-Rodgers, Helen F.


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