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dc.contributor.authorSarkar, Sohinee
dc.contributor.authorUlett, Glen C
dc.contributor.authorTotsika, Makrina
dc.contributor.authorMinh-Duy, Phan
dc.contributor.authorSchembri, Mark A
dc.date.accessioned2017-05-03T15:29:39Z
dc.date.available2017-05-03T15:29:39Z
dc.date.issued2014
dc.date.modified2014-08-08T04:06:40Z
dc.identifier.issn1932-6203
dc.identifier.doi10.1371/journal.pone.0094786
dc.identifier.urihttp://hdl.handle.net/10072/62074
dc.description.abstractUrinary tract infection (UTI) is one of the most common bacterial infections in humans, with uropathogenic Escherichia coli (UPEC) the leading causative organism. UPEC has a number of virulence factors that enable it to overcome host defenses within the urinary tract and establish infection. The O antigen and the capsular polysaccharide are two such factors that provide a survival advantage to UPEC. Here we describe the application of the rpsL counter selection system to construct capsule (kpsD) and O antigen (waaL) mutants and complemented derivatives of three reference UPEC strains: CFT073 (O6:K2:H1), RS218 (O18:K1:H7) and 1177 (O1:K1:H7). We observed that while the O1, O6 and O18 antigens were required for survival in human serum, the role of the capsule was less clear and linked to O antigen type. In contrast, both the K1 and K2 capsular antigens provided a survival advantage to UPEC in whole blood. In the mouse urinary tract, mutation of the O6 antigen significantly attenuated CFT073 bladder colonization. Overall, this study contrasts the role of capsule and O antigen in three common UPEC serotypes using defined mutant and complemented strains. The combined mutagenesis-complementation strategy can be applied to study other virulence factors with complex functions both in vitro and in vivo.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent971411 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherPublic Library of Science
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationY
dc.relation.ispartofpagefrome94786-1
dc.relation.ispartofpagetoe94786-11
dc.relation.ispartofissue4
dc.relation.ispartofjournalPloS One
dc.relation.ispartofvolume9
dc.rights.retentionN
dc.subject.fieldofresearchMedical bacteriology
dc.subject.fieldofresearchcode320701
dc.titleRole of Capsule and O Antigen in the Virulence of Uropathogenic Escherichia coli
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://www.plos.org/journals/license.html
gro.rights.copyright© 2014 Sarkar et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License CCAL. (http://www.plos.org/journals/license.html)
gro.hasfulltextFull Text
gro.griffith.authorUlett, Glen C.


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