Show simple item record

dc.contributor.authorSun, Yi
dc.contributor.authorNguyen, Nam-Trung
dc.contributor.authorKwok, Yien Chian
dc.date.accessioned2017-05-03T12:44:58Z
dc.date.available2017-05-03T12:44:58Z
dc.date.issued2008
dc.date.modified2014-08-13T23:52:27Z
dc.identifier.issn0003-2700
dc.identifier.doi10.1021/ac800787g
dc.identifier.urihttp://hdl.handle.net/10072/62206
dc.description.abstractWe report here a novel multichannel closed-loop magnetically actuated microchip for high-throughput polymerase chain reaction (PCR). This is achieved by designing a series of concentric circular channels on one microchip and exploiting a magnetic force to drive DNA samples flowing continuously through the closed loops. The magnetic force arises from an external permanent magnet through ferrofluid plugs inside the microchannels. The magnet enables simultaneous actuation of DNA samples in all the channels. As the samples go around the loops, they pass through three preset temperature zones. Parameters of PCR, such as incubation time, temperatures, and number of cycles, can be fully controlled and adjusted. High reproducibility was achieved for different channels in the same run and for the same channels in consecutive runs. Genetically modified organisms (GMOs) were amplified simultaneously using the developed device. This simple, reliable, and high-throughput PCR microchip would find wide applications in forensic, clinical, and biological fields.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.publisherAmerican Chemical Society
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom6127
dc.relation.ispartofpageto6130
dc.relation.ispartofissue15
dc.relation.ispartofjournalAnalytical Chemistry
dc.relation.ispartofvolume80
dc.rights.retentionY
dc.subject.fieldofresearchAnalytical Chemistry not elsewhere classified
dc.subject.fieldofresearchAnalytical Chemistry
dc.subject.fieldofresearchChemical Engineering
dc.subject.fieldofresearchOther Chemical Sciences
dc.subject.fieldofresearchcode030199
dc.subject.fieldofresearchcode0301
dc.subject.fieldofresearchcode0904
dc.subject.fieldofresearchcode0399
dc.titleHigh-throughput polymerase chain reaction in parallel circular loops using magnetic actuation
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyrightSelf-archiving of the author-manuscript version is not yet supported by this journal. Please refer to the journal link for access to the definitive, published version or contact the authors for more information.
gro.hasfulltextNo Full Text
gro.griffith.authorNguyen, Nam-Trung


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record