A Sco homologue plays a role in defence against oxidative stress in pathogenic Neisseria.
Abstract
Sco proteins are found in mitochondria and in a variety of oxidase positive bacteria. Although Sco is required for the formation of the CuA centre in a cytochrome oxidase of the aa3 type, it was observed that oxidases with a CuA centre are not present in many bacteria that contain a Sco homologue. Two bacteria of this type are the pathogens Neisseria meningitidis and Neisseria gonorrhoeae. The sco genes of N. gonorrhoeae strain 1291 and N. meningitidis strain MC58 were cloned, inactivated by inserting a kanamycin resistance cassette and used to make knockout mutants by allelic exchange. Both N. gonorrhoeae and N. meningitidis ...
View more >Sco proteins are found in mitochondria and in a variety of oxidase positive bacteria. Although Sco is required for the formation of the CuA centre in a cytochrome oxidase of the aa3 type, it was observed that oxidases with a CuA centre are not present in many bacteria that contain a Sco homologue. Two bacteria of this type are the pathogens Neisseria meningitidis and Neisseria gonorrhoeae. The sco genes of N. gonorrhoeae strain 1291 and N. meningitidis strain MC58 were cloned, inactivated by inserting a kanamycin resistance cassette and used to make knockout mutants by allelic exchange. Both N. gonorrhoeae and N. meningitidis sco mutants were highly sensitive to oxidative killing by paraquat, indicating that Sco is involved in protection against oxidative stress in these bacteria.
View less >
View more >Sco proteins are found in mitochondria and in a variety of oxidase positive bacteria. Although Sco is required for the formation of the CuA centre in a cytochrome oxidase of the aa3 type, it was observed that oxidases with a CuA centre are not present in many bacteria that contain a Sco homologue. Two bacteria of this type are the pathogens Neisseria meningitidis and Neisseria gonorrhoeae. The sco genes of N. gonorrhoeae strain 1291 and N. meningitidis strain MC58 were cloned, inactivated by inserting a kanamycin resistance cassette and used to make knockout mutants by allelic exchange. Both N. gonorrhoeae and N. meningitidis sco mutants were highly sensitive to oxidative killing by paraquat, indicating that Sco is involved in protection against oxidative stress in these bacteria.
View less >
Journal Title
FEBS Letters
Volume
546
Issue
2-3
Subject
Bacteriology
Medicinal and Biomolecular Chemistry
Biochemistry and Cell Biology
Evolutionary Biology