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dc.contributor.authorAlfredson, DA
dc.contributor.authorKorolik, V
dc.contributor.editorD. K. Chattoraj
dc.date.accessioned2017-05-03T11:42:36Z
dc.date.available2017-05-03T11:42:36Z
dc.date.issued2003
dc.identifier.issn0147-619X
dc.identifier.doi10.1016/S0147-619X(03)00060-X
dc.identifier.urihttp://hdl.handle.net/10072/6230
dc.description.abstractA small cryptic plasmid, pCJ419, was identified in a human clinical isolate of Campylobacter jejuni, cloned and sequenced. pCJ419 is a circular molecule of 4013 bp with a G + C content of 27.1%. The products of four open reading frames (ORFs) share significant sequence similarity with putative proteins from known C. jejuni and Campylobacter coli plasmids. ORF-1 encodes a putative mobilisation protein (Mob). ORF-2 and ORF-3 encode proteins that have high identity to putative RepA and RepB proteins, respectively, of known C. jejuni and C. coli plasmids. ORF-4 encodes a protein that has high identity to a hypothetical protein of unknown function, Cjp32, previously described in a pVir plasmid of C. jejuni. Tandem repeating 22-bp sequences typical of a plasmid replication origin (ori) were identified upstream of the DNA sequences encoding putative replication initiation proteins. An Escherichia coli-Campylobacter shuttle cloning vector, pGU0202, was constructed using plasmid pMW2 that harbours a Campylobacter-derived kanamycin resistance gene [aph(3')-III]. The sequences encoding pCJ419 mob, RepA and RepB proteins were inserted upstream of aph(3')-III resulting in a stable construct of 6174 bp that was used to transform both E. coli and Campylobacter.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoen_US
dc.publisherAcademic Press inc. Elsevier Science
dc.publisher.placeSan Diego, USA
dc.relation.ispartofpagefrom152
dc.relation.ispartofpageto160
dc.relation.ispartofjournalPlasmid
dc.relation.ispartofvolume50
dc.subject.fieldofresearchGenetics
dc.subject.fieldofresearchcode0604
dc.titleSequence analysis of a cryptic plasmid pCJ419 from Campylobacter jejuni and construction of an Escherichia coli-Campylobacter shuttle vector.
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Health, School of Medical Science
gro.date.issued2015-05-06T21:36:40Z
gro.hasfulltextNo Full Text
gro.griffith.authorKorolik, Victoria
gro.griffith.authorAlfredson, David


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