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dc.contributor.authorHuebsch, Z
dc.contributor.authorVan Zyl, RL
dc.contributor.authorCock, IE
dc.contributor.authorVan Vuuren, SF
dc.date.accessioned2017-05-03T11:28:50Z
dc.date.available2017-05-03T11:28:50Z
dc.date.issued2014
dc.date.modified2014-08-19T04:41:00Z
dc.identifier.issn0254-6299
dc.identifier.doi10.1016/j.sajb.2014.04.005
dc.identifier.urihttp://hdl.handle.net/10072/62479
dc.description.abstractMedicinal plant use plays an important role in the healthcare of many South Africans. Furthermore, in orthodox medicine, conventional antimicrobial agents are amongst themost commonly prescribed groups of drugs. Therefore, due to the prevalence of use of these two forms of healthcare, there is a high probability for their concurrent use. Thus, the aimof this study was to evaluate the interactive antimicrobial and toxicity profiles of six Southern African medicinal plants (Agathosma betulina, Aloe ferox, Artemisia afra, Lippia javanica, Pelargonium sidoides and Sutherlandia frutescens) when combined with seven conventional antimicrobials (ciprofloxacin, erythromycin, gentamicin, penicillin G, tetracycline, amphotericin B and nystatin). Antimicrobial activity was assessed using the minimum inhibitory concentration (MIC) assay against a range of pathogens and interactions were further classified using the sum of the fractional inhibitory concentration (SFIC). Notable synergistic or antagonistic interactions were studied at various ratios (isobolograms). The toxicity of the individual samples, aswell as the notable combinations, was assessed using the brine-shrimp lethality assay (BSLA) and the 3-(4,5 dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on the HEK-293 human cell line. Of the 420 antimicrobial: plant combinations studied, 14.29% showed synergistic interactions, 7.56% antagonistic, 35.71% additive and 42.44% indifferent interactions. Some notable synergistic interactions (ciprofloxacin with A. betulina and S. frutescens against Escherichia coli) and antagonistic interactions (ciprofloxacin with A. afra organic extract against Escherichia coli) were identified. None of the notable combinations were found to show toxicity in the BSLA or MTT assay. In conclusion, the majority of combinations were found to have no notable interaction, alleviating some concern related to the concurrent use of these two forms of healthcare.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.publisher.placeNetherlands
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom185
dc.relation.ispartofpageto197
dc.relation.ispartofjournalSouth African Journal of Botany
dc.relation.ispartofvolume93
dc.rights.retentionY
dc.subject.fieldofresearchEcology
dc.subject.fieldofresearchPlant biology
dc.subject.fieldofresearchTraditional, complementary and integrative medicine not elsewhere classified
dc.subject.fieldofresearchcode3103
dc.subject.fieldofresearchcode3108
dc.subject.fieldofresearchcode420899
dc.titleInteractive antimicrobial and toxicity profiles of conventional antimicrobials with Southern African medicinal plants
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorCock, Ian E.


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