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dc.contributor.authorMurcia, Pablo R.
dc.contributor.authorHughes, Joseph
dc.contributor.authorBattista, Patrizia
dc.contributor.authorLloyd, Lucy
dc.contributor.authorBaillie, Gregory J.
dc.contributor.authorRamirez-Gonzalez, Ricardo H.
dc.contributor.authorOrmond, Doug
dc.contributor.authorOliver, Karen
dc.contributor.authorElton, Debra
dc.contributor.authorMumford, Jennifer A.
dc.contributor.authorCaccamo, Mario
dc.contributor.authorKellam, Paul
dc.contributor.authorGrenfell, Bryan T.
dc.contributor.authorHolmes, Edward C.
dc.contributor.authorWood, James L. N.
dc.date.accessioned2018-01-12T01:43:32Z
dc.date.available2018-01-12T01:43:32Z
dc.date.issued2012
dc.date.modified2014-08-19T04:43:16Z
dc.identifier.issn1553-7366
dc.identifier.doi10.1371/journal.ppat.1002730
dc.identifier.urihttp://hdl.handle.net/10072/62540
dc.description.abstractInfluenza viruses are characterized by an ability to cross species boundaries and evade host immunity, sometimes with devastating consequences. The 2009 pandemic of H1N1 influenza A virus highlights the importance of pigs in influenza emergence, particularly as intermediate hosts by which avian viruses adapt to mammals before emerging in humans. Although segment reassortment has commonly been associated with influenza emergence, an expanded host-range is also likely to be associated with the accumulation of specific beneficial point mutations. To better understand the mechanisms that shape the genetic diversity of avian-like viruses in pigs, we studied the evolutionary dynamics of an Eurasian Avian-like swine influenza virus (EA-SIV) in naı¨ve and vaccinated pigs linked by natural transmission. We analyzed multiple clones of the hemagglutinin 1 (HA1) gene derived from consecutive daily viral populations. Strikingly, we observed both transient and fixed changes in the consensus sequence along the transmission chain. Hence, the mutational spectrum of intra-host EA-SIV populations is highly dynamic and allele fixation can occur with extreme rapidity. In addition, mutations that could potentially alter host-range and antigenicity were transmitted between animals and mixed infections were commonplace, even in vaccinated pigs. Finally, we repeatedly detected distinct stop codons in virus samples from co-housed pigs, suggesting that they persisted within hosts and were transmitted among them. This implies that mutations that reduce viral fitness in one host, but which could lead to fitness benefits in a novel host, can circulate at low frequencies.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherPublic Library of Science
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrome1002730-1
dc.relation.ispartofpagetoe1002730-12
dc.relation.ispartofissue5
dc.relation.ispartofjournalPLoS Pathogens
dc.relation.ispartofvolume8
dc.rights.retentionY
dc.subject.fieldofresearchMedical Microbiology not elsewhere classified
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchImmunology
dc.subject.fieldofresearchMedical Microbiology
dc.subject.fieldofresearchcode110899
dc.subject.fieldofresearchcode0605
dc.subject.fieldofresearchcode1107
dc.subject.fieldofresearchcode1108
dc.titleEvolution of an Eurasian Avian-like Influenza Virus in Naïve and Vaccinated Pigs
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttps://creativecommons.org/licenses/by/4.0/
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2012 Murcia et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
gro.hasfulltextFull Text
gro.griffith.authorBaillie, Greg


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