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dc.contributor.authorTan, Tien-Chye
dc.contributor.authorY. Yien, Yvette
dc.contributor.authorPatel, Bharat
dc.contributor.authorMijts, Benjamin
dc.contributor.authorSwaminathan, Kunchithapadam
dc.contributor.editorC E Bugg
dc.date.accessioned2017-05-03T11:40:29Z
dc.date.available2017-05-03T11:40:29Z
dc.date.issued2003
dc.date.modified2009-09-16T07:41:23Z
dc.identifier.issn09074449
dc.identifier.doi10.1107/S0907444903018754
dc.identifier.urihttp://hdl.handle.net/10072/6289
dc.description.abstractThis is a report on the structure determination of AmyB, the second a-amylase from Halothermothrix orenii, by X-ray crystallography. This bacterium was isolated from saltpans where conditions consisted of both high temperatures and high NaCl content. AmyB is a 599-�sidue protein which is stable and significantly active at 358 K in starch solution containing up to 10%(w/v) NaCl. The purified recombinant AmyB protein crystallizes in the monoclinic space group C2, with unit-cell parameters a = 225.85, b = 77.16, c = 50.13 Ŭ ߠ= 99.32ଠusing the hanging-drop vapour-diffusion method. The crystal diffracts X-�ys to a resolution limit of 1.97 Ů
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherBlackwell Munksgaard
dc.publisher.placeDenmark
dc.publisher.urihttp://www.blackwell-synergy.com/doi/full/10.1107/S0907444903018754
dc.relation.ispartofpagefrom2257
dc.relation.ispartofpageto2258
dc.relation.ispartofjournalActa Crystallographica, Section D: Biological Crystallography
dc.relation.ispartofvolumeD59
dc.subject.fieldofresearchPhysical Sciences
dc.subject.fieldofresearchChemical Sciences
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchcode02
dc.subject.fieldofresearchcode03
dc.subject.fieldofresearchcode06
dc.titleCrystallization of a novel a-amylase, AmyB, from the thermophilic halophile Halothermothrix orenii
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2003
gro.hasfulltextNo Full Text
gro.griffith.authorPatel, Bharat K.
gro.griffith.authorMijts, Benjamin


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