dc.contributor.author | Tan, Tien-Chye | |
dc.contributor.author | Y. Yien, Yvette | |
dc.contributor.author | Patel, Bharat | |
dc.contributor.author | Mijts, Benjamin | |
dc.contributor.author | Swaminathan, Kunchithapadam | |
dc.contributor.editor | C E Bugg | |
dc.date.accessioned | 2017-05-03T11:40:29Z | |
dc.date.available | 2017-05-03T11:40:29Z | |
dc.date.issued | 2003 | |
dc.date.modified | 2009-09-16T07:41:23Z | |
dc.identifier.issn | 09074449 | |
dc.identifier.doi | 10.1107/S0907444903018754 | |
dc.identifier.uri | http://hdl.handle.net/10072/6289 | |
dc.description.abstract | This is a report on the structure determination of AmyB, the second a-amylase from Halothermothrix orenii, by X-ray crystallography. This bacterium was isolated from saltpans where conditions consisted of both high temperatures and high NaCl content. AmyB is a 599-�sidue protein which is stable and significantly active at 358 K in starch solution containing up to 10%(w/v) NaCl. The purified recombinant AmyB protein crystallizes in the monoclinic space group C2, with unit-cell parameters a = 225.85, b = 77.16, c = 50.13 Ŭ ߠ= 99.32ଠusing the hanging-drop vapour-diffusion method. The crystal diffracts X-�ys to a resolution limit of 1.97 Ů | |
dc.description.peerreviewed | Yes | |
dc.description.publicationstatus | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Blackwell Munksgaard | |
dc.publisher.place | Denmark | |
dc.publisher.uri | http://www.blackwell-synergy.com/doi/full/10.1107/S0907444903018754 | |
dc.relation.ispartofpagefrom | 2257 | |
dc.relation.ispartofpageto | 2258 | |
dc.relation.ispartofjournal | Acta Crystallographica, Section D: Biological Crystallography | |
dc.relation.ispartofvolume | D59 | |
dc.subject.fieldofresearch | Physical Sciences | |
dc.subject.fieldofresearch | Chemical Sciences | |
dc.subject.fieldofresearch | Biological Sciences | |
dc.subject.fieldofresearchcode | 02 | |
dc.subject.fieldofresearchcode | 03 | |
dc.subject.fieldofresearchcode | 06 | |
dc.title | Crystallization of a novel a-amylase, AmyB, from the thermophilic halophile Halothermothrix orenii | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.date.issued | 2003 | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Patel, Bharat K. | |
gro.griffith.author | Mijts, Benjamin | |