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dc.contributor.authorBloomfield, KL
dc.contributor.authorOsborne, SA
dc.contributor.authorKennedy, DD
dc.contributor.authorClarke, FM
dc.contributor.authorTonissen, KF
dc.contributor.editorG. Bernardi, Alberto Bernardi
dc.date.accessioned2017-05-03T13:22:16Z
dc.date.available2017-05-03T13:22:16Z
dc.date.issued2003
dc.date.modified2009-12-07T03:32:38Z
dc.identifier.issn0378-1119
dc.identifier.doi10.1016/S0378-1119(03)00799-6
dc.identifier.urihttp://hdl.handle.net/10072/6323
dc.description.abstractIn recent years, redox control has emerged as a fundamental mechanism of gene regulation through transcriptional control. Thioredoxin (Trx) is a dithiol-reducing enzyme known to be involved in the redox regulation of a number of transcription factors, and in this study, we have investigated the redox-dependent regulation of the DNA binding activity of Sp1 by thioredoxin. Electrophoretic mobility shift assays were used to show that both recombinant Sp1 produced in Escherichia coli and endogenous Sp1 expressed by MDA-MB-231 breast cancer cells is subject to redox regulation. We found that thioredoxin alone or in conjunction with the full thioredoxin system (comprising thioredoxin, thioredoxin reductase [TR], and a-nicotinamide adenine dinucleotide phosphate [NADPH]) can increase Sp1 DNA binding activity in vitro to an oligonucleotide containing the Sp1 consensus sequence. Furthermore, we have provided evidence that recombinant Sp1 can bind to Sp1 consensus sequences within a 330-base pair (bp) thioredoxin promoter fragment and that this interaction can also be enhanced by the presence of thioredoxin. Luciferase reporter assays using this same minimal thioredoxin promoter region demonstrated that both Sp1 and Sp3 can bind to the promoter and act to enhance transcription. When the three identified Sp1 consensus sequences within the reporter construct were deleted, there was a loss of basal promoter activity, showing that these closely positioned sites are important for regulation of thioredoxin gene expression.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoen_AU
dc.publisherElsevier Science
dc.publisher.placeNetherlands
dc.publisher.urihttp://www.elsevier.com/wps/find/journaldescription.cws_home/506033/description#description
dc.relation.ispartofpagefrom107
dc.relation.ispartofpageto116
dc.relation.ispartofedition2003
dc.relation.ispartofjournalGene: an international journal focusing on gene cloning and gene structure and function
dc.relation.ispartofvolume319
dc.subject.fieldofresearchGenetics
dc.subject.fieldofresearchPhysiology
dc.subject.fieldofresearchMedical Microbiology
dc.subject.fieldofresearchcode0604
dc.subject.fieldofresearchcode0606
dc.subject.fieldofresearchcode1108
dc.titleThioredoxin-mediated redox control of the transcription factor Sp1 and regulation of the thioredoxin gene promoter
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyright© 2003 Elsevier : Reproduced in accordance with the copyright policy of the publisher : This journal is available online - use hypertext links.
gro.date.issued2003
gro.hasfulltextNo Full Text
gro.griffith.authorClarke, Francis M.
gro.griffith.authorKennedy, Derek D.
gro.griffith.authorMcGrath, Kelly
gro.griffith.authorTonissen, Kathryn F.
gro.griffith.authorOsborne, Simone A.


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