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dc.contributor.authorDashti, Yousef
dc.contributor.authorGrkovic, Tanja
dc.contributor.authorAbdelmohsen, Usama Ramadan
dc.contributor.authorHentschel, Ute
dc.contributor.authorQuinn, Ronald J
dc.date.accessioned2017-05-03T15:59:45Z
dc.date.available2017-05-03T15:59:45Z
dc.date.issued2014
dc.identifier.issn1660-3397
dc.identifier.doi10.3390/md12053046
dc.identifier.urihttp://hdl.handle.net/10072/63712
dc.description.abstractTwo sponge-derived actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163, were grown in co-culture and the presence of induced metabolites monitored by 1H NMR. Ten known compounds, including angucycline, diketopiperazine and ߭carboline derivatives 1-10, were isolated from the EtOAc extracts of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163. Co-cultivation of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163 induced the biosynthesis of three natural products that were not detected in the single culture of either microorganism, namely N-(2-hydroxyphenyl)-acetamide (11), 1,6-dihydroxyphenazine (12) and 5a,6,11a,12-tetrahydro-5a,11a-dimethyl[1,4]benzoxazino[3,2-b][1,4]benzoxazine (13a). When tested for biological activity against a range of bacteria and parasites, only the phenazine 12 was active against Bacillus sp. P25, Trypanosoma brucei and interestingly, against Actinokineospora sp. EG49. These findings highlight the co-cultivation approach as an effective strategy to access the bioactive secondary metabolites hidden in the genomes of marine actinomycetes.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent792802 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherM D P I AG
dc.publisher.placeSwitzerland
dc.relation.ispartofstudentpublicationY
dc.relation.ispartofpagefrom3046
dc.relation.ispartofpageto3059
dc.relation.ispartofissue5
dc.relation.ispartofjournalMarine Drugs
dc.relation.ispartofvolume12
dc.rights.retentionY
dc.subject.fieldofresearchBiologically active molecules
dc.subject.fieldofresearchPhysical chemistry
dc.subject.fieldofresearchPharmacology and pharmaceutical sciences
dc.subject.fieldofresearchcode340401
dc.subject.fieldofresearchcode3406
dc.subject.fieldofresearchcode3214
dc.titleProduction of Induced Secondary Metabolites by a Co-Culture of Sponge-Associated Actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by/3.0/
gro.facultyGriffith Sciences, Griffith Institute for Drug Discovery
gro.rights.copyright© 2014 by the authors; licensee MDPI, author. This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported (CC BY 3.0) License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorQuinn, Ronald J.
gro.griffith.authorGrkovic, Tanja
gro.griffith.authorDashti, Yousef


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