High throughput extraction of plasma using a secondary flow-aided inertial microfluidic device
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Author(s)
Zhang, Jun
Yan, Sheng
Li, Weihua
Alici, Gursel
Nam-Trung, Nguyen
Year published
2014
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In this paper, we report the development of a simple inertial microfluidic device with a serpentine channel for efficiently separating blood cells from plasma. The working mechanism of this device relies on the two-sided secondary flow aided inertial focusing of particles in a serpentine channel. Specifically, blood cells were focused along two sides of the channel, while the blood plasma was collected at the cell-free region within the channel centre. The device was tested with diluted (1/20) whole blood. A relatively high flow rate of 350 嬠min-1 with a purity of [similar]99.75% was achieved in a single process. A further ...
View more >In this paper, we report the development of a simple inertial microfluidic device with a serpentine channel for efficiently separating blood cells from plasma. The working mechanism of this device relies on the two-sided secondary flow aided inertial focusing of particles in a serpentine channel. Specifically, blood cells were focused along two sides of the channel, while the blood plasma was collected at the cell-free region within the channel centre. The device was tested with diluted (1/20) whole blood. A relatively high flow rate of 350 嬠min-1 with a purity of [similar]99.75% was achieved in a single process. A further improvement to 99.95% purity was obtained after a second process. Parallelization with eight parallel serpentine channels achieved a high flow rate of 2.8 ml min-1 and a massive throughput of 7 נ108 cells per min. Our device could be easily integrated with other sample preparation processes or detection units to form a sample-to-answer lab-on-a-chip system.
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View more >In this paper, we report the development of a simple inertial microfluidic device with a serpentine channel for efficiently separating blood cells from plasma. The working mechanism of this device relies on the two-sided secondary flow aided inertial focusing of particles in a serpentine channel. Specifically, blood cells were focused along two sides of the channel, while the blood plasma was collected at the cell-free region within the channel centre. The device was tested with diluted (1/20) whole blood. A relatively high flow rate of 350 嬠min-1 with a purity of [similar]99.75% was achieved in a single process. A further improvement to 99.95% purity was obtained after a second process. Parallelization with eight parallel serpentine channels achieved a high flow rate of 2.8 ml min-1 and a massive throughput of 7 נ108 cells per min. Our device could be easily integrated with other sample preparation processes or detection units to form a sample-to-answer lab-on-a-chip system.
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Journal Title
RSC Advances
Volume
4
Copyright Statement
© 2014 Royal Society of Chemistry. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal website for access to the definitive, published version.
Subject
Chemical sciences
Biomedical instrumentation